This transgenic mouse line, with an "enhanced" GFP (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer, have widespread EGFP fluorescence, with the exception of erythrocytes and hair.
Dr. Masaru Okabe, Osaka University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
This transgenic mouse line, with an "enhanced" GFP (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer, have widespread EGFP fluorescence, with the exception of erythrocytes and hair. Note that mice homozygous for this transgene die within the first two weeks following birth. Mice homozygous for this transgene on the C57BL/6 background have a perinatal lethal phenotype.
Of note, it has been the experience at The Jackson Laboratory that Stock No. 006567 (C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ) demonstrates the highest proportion of GFP expressing cell populations in bone marrow, thymus, spleen and peripheral blood when compared to Stock No. 003291 (C57BL/6-Tg(CAG-EGFP)1Osb/J) and Stock No. 007075 (CByJ.B6-Tg(CAG-EGFP)1Osb/J).
View the pdf document on GFP Expressing Lymphocyte Populations in C57BL/6-Tg(CAG-EGFP)1Osb/J, C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ and CByJ.B6-Tg(CAG-EGFP)1Osb/J.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the Stock No. 007075. It should be noted that the phenotype could vary from that originally described for this transgene on C57BL/6 (Stock No. 003291). We will modify the strain description if necessary as published results become available.
The EcoRI sites included in the PCR primers were used to introduce the amplified EGFP cDNA into a pCAGGS expression vector containing the chicken beta-actin promoter and cytomegalovirus enhancer, beta-actin intron and bovine globin poly-adenylation signal. The entire insert with the promoter and coding sequence was excised with Bam-HI and SalI and gel-purified. This strain was generated in C57BL/6 mice. The mice were then backcrossed to BALB/cByJ (Stock No. 001026) using a speed congenic protocol.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | All tissues, with the exception of erythrocytes and hair, appear green under excitation light. |
Allele Name | transgene insertion 1, Research Institute for Microbial Diseases, Osaka University |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | (ACTbEGFP)10sb; beta-actin-GFP; GFP; pCAGGS-GFP; Ten(ACTbEGFP)1Osb; Tg(ACTbEGFP)1Osb; Tg(ACTB-EGFP)1Osb; Tg(CAG-EGFP)1Osb; TgN(ACTbEGFP)1Osb; TgN(beta-act-EFGP)01Osb |
Gene Symbol and Name | Tg(CAG-EGFP)1Osb, transgene insertion 1, Research Institute for Microbial Diseases, Osaka University |
Gene Synonym(s) | |
Promoter | CAG, CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | All tissues, with the exception of erythrocytes and hair, appear green under excitation light. |
Strain of Origin | C57BL/6 |
Chromosome | 15 |
Molecular Note | cDNA encoding enhanced green fluorescent protein (EGFP) was adjoined to the chicken beta actin promoter and cytomegalovirus enhancer. A bovine globin polyadenylation signal was also included in the construct. |
Mutations Made By | Dr. Masaru Okabe, Osaka University |
When maintaining a live colony, hemizygous mice may be bred to wildtype siblings, or to BALB/cByJ inbred mice (Stock No. 001026). Mice homozygous for this transgene on the C57BL/6 background have a perinatal lethal phenotype.
When using the CByJ.B6 ACTb-EGFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #007075 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(CAG-EGFP)1Osb |
Frozen Mouse Embryo | CByJ.B6-Tg(CAG-EGFP)1Osb/J | $2595.00 |
Frozen Mouse Embryo | CByJ.B6-Tg(CAG-EGFP)1Osb/J | $2595.00 |
Frozen Mouse Embryo | CByJ.B6-Tg(CAG-EGFP)1Osb/J | $3373.50 |
Frozen Mouse Embryo | CByJ.B6-Tg(CAG-EGFP)1Osb/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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