CByJ.B6-Tg(CAG-EGFP)1Osb/J

Stock number: 007075
Product name: CByJ.B6 ACTb-EGFP
Research areas: Research Tools

Description

This transgenic mouse line, with an "enhanced" GFP (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer, have widespread EGFP fluorescence, with the exception of erythrocytes and hair.

Detailed description

This transgenic mouse line, with an "enhanced" GFP (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer, have widespread EGFP fluorescence, with the exception of erythrocytes and hair. Note that mice homozygous for this transgene die within the first two weeks following birth. Mice homozygous for this transgene on the C57BL/6 background have a perinatal lethal phenotype.

Of note, it has been the experience at The Jackson Laboratory that Stock No. 006567 (C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ) demonstrates the highest proportion of GFP expressing cell populations in bone marrow, thymus, spleen and peripheral blood when compared to Stock No. 003291 (C57BL/6-Tg(CAG-EGFP)1Osb/J) and Stock No. 007075 (CByJ.B6-Tg(CAG-EGFP)1Osb/J).

View the pdf document on GFP Expressing Lymphocyte Populations in C57BL/6-Tg(CAG-EGFP)1Osb/J, C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ and CByJ.B6-Tg(CAG-EGFP)1Osb/J.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the Stock No. 007075. It should be noted that the phenotype could vary from that originally described for this transgene on C57BL/6 (Stock No. 003291). We will modify the strain description if necessary as published results become available.

Development

The EcoRI sites included in the PCR primers were used to introduce the amplified EGFP cDNA into a pCAGGS expression vector containing the chicken beta-actin promoter and cytomegalovirus enhancer, beta-actin intron and bovine globin poly-adenylation signal. The entire insert with the promoter and coding sequence was excised with Bam-HI and SalI and gel-purified. This strain was generated in C57BL/6 mice. The mice were then backcrossed to BALB/cByJ (Stock No. 001026) using a speed congenic protocol.

Allele

Symbol: Tg(CAG-EGFP)1Osb
Name: transgene insertion 1, Research Institute for Microbial Diseases, Osaka University
MGI accession ID: MGI:2686773
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(CAG-EGFP)1Osb
Marker name: transgene insertion 1, Research Institute for Microbial Diseases, Osaka University
Chromosome: 15
Strain of origin: C57BL/6
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: All tissues, with the exception of erythrocytes and hair, appear green under excitation light.
Molecular note: cDNA encoding enhanced green fluorescent protein (EGFP) was adjoined to the chicken beta actin promoter and cytomegalovirus enhancer. A bovine globin polyadenylation signal was also included in the construct.