Mice homozygous for this Cd40lg (CD40 ligand), Cd40lg tm1Imx, targeted mutation may be useful for studying immune system physiology, response to prion infection, neurodegeneration, apoptosis and Immunodeficiency with Hyper-IgM, Type 1.
IMR Colony, The Jackson Laboratory
Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice show no overt phenotypic abnormalities. Percentages of B and T cell subpopulations is normal. Homozygotes do show selective deficiencies in humoral immunity (low basal serum isotype levels and undetectable IgE) as well as abnormal secondary antigen-specific responses to immunization with a thymus-dependent antigen. The phenotype of the mice resembles human X-linked hyper IgM syndrome. This mutant mouse strain may be useful in studies of immune system physiology, response to prion infection, neurodegeneration, apoptosis and Immunodeficiency with Hyper-IgM, Type 1.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector containing a PGKneo cassette was used to replace exons 3 and 4. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting male chimeric animals were crossed to C57BL/6 female mice. Heterozygous (129/SV X C57BL/6)F1 females were crossed to C57BL/6 males to generate hemizygous males. The mice were subsequently backcrossed on to the C57BL/6J (and/or C57BL/6N) genetic background for five generations. The mice were next backcrossed to BALB/cByJ (Stock No. 001026) using a speed congenic protocol to produce this strain.
|Allele Name||targeted mutation 1, Immunex|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||CD154-; CD40L-; CD40LKO; Tnfsf5tm1Imx|
|Gene Symbol and Name||Cd40lg, CD40 ligand|
|Strain of Origin||129S2/SvPas|
|Molecular Note||Exons 3 and 4 were replaced with a neomycin cassette. Homozygous mutant animals fail to express functional protein on the cell surface.|
|Mutations Made By|| |
Dr. Jacques Peschon, Amgen
When maintaining a live colony, these mice can be bred as homozygous females and hemizygous males. The targeted mutation is X-linked. These mice are somewhat immunocompromised, specific pathogen-free (SPF) conditions are recommended.
When using the CD40 ligand KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #007074 in your Materials and Methods section.