Mice homozygous for this Cd40lg (CD40 ligand), Cd40lg tm1Imx, targeted mutation may be useful for studying immune system physiology, response to prion infection, neurodegeneration, apoptosis and Immunodeficiency with Hyper-IgM, Type 1.
IMR Colony, The Jackson Laboratory
Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice show no overt phenotypic abnormalities. Percentages of B and T cell subpopulations is normal. Homozygotes do show selective deficiencies in humoral immunity (low basal serum isotype levels and undetectable IgE) as well as abnormal secondary antigen-specific responses to immunization with a thymus-dependent antigen. The phenotype of the mice resembles human X-linked hyper IgM syndrome. This mutant mouse strain may be useful in studies of immune system physiology, response to prion infection, neurodegeneration, apoptosis and Immunodeficiency with Hyper-IgM, Type 1.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector containing a PGKneo cassette was used to replace exons 3 and 4. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting male chimeric animals were crossed to C57BL/6 female mice. Heterozygous (129/SV X C57BL/6)F1 females were crossed to C57BL/6 males to generate hemizygous males. The mice were subsequently backcrossed on to the C57BL/6J (and/or C57BL/6N) genetic background for five generations. The mice were next backcrossed to BALB/cByJ (Stock No. 001026) using a speed congenic protocol to produce this strain.
|Allele Name||targeted mutation 1, Immunex|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||CD154-; CD40L-; CD40LKO; Tnfsf5tm1Imx|
|Gene Symbol and Name||Cd40lg, CD40 ligand|
|Gene Synonym(s)||CD154; CD40 antigen ligand; CD40L; Cd40L; Cd40l; Cd40l; HIGM1; IGM; IMD3; Ly-62; Ly-62; Ly62; T-BAM; TNFSF5; TRAP; Tnfsf5; Tnfsf5; gp39; hCD40L; lymphocyte antigen 62; tumor necrosis factor (ligand) superfamily, member 5|
|Strain of Origin||129S2/SvPas|
|Molecular Note||Exons 3 and 4 were replaced with a neomycin cassette. Homozygous mutant animals fail to express functional protein on the cell surface.|
|Mutations Made By|| |
Dr. Jacques Peschon, Amgen
When maintaining a live colony, these mice can be bred as homozygous females and hemizygous males. The targeted mutation is X-linked. These mice are somewhat immunocompromised, specific pathogen-free (SPF) conditions are recommended.
|Please inquire about possible genotypes.|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in
order to provide the minimum number of animals, animals will ship within 25 weeks.
The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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