Mice homozygous for this Ldlr (low density lipoprotein receptor) targeted mutation, Ldlrtm1Her, may be useful for studying lipid and leptin homeostasis, hyperglycemia, hypercholesterolemia, atherosclerosis, and the effects of diet on skin and coat.Read More +
Mice homozygous for the Ldlrtm1Her mutation have an elevated serum cholesterol level of 200-400 mg/dl and they have very high levels (>2,000 mg/dl) when fed a high fat diet. Normal serum cholesterol in the mouse is 80-100 mg/dl. This mutant mouse strain may be useful in studies of lipid and leptin homeostasis, hyperglycemia, hypercholesterolemia, atherosclerosis, and the effects of diet on skin and coat.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 4. The construct was electroporated into 129S7/SvEvBrd-Hprt1+ derived AB1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice, and then backcrossed to C57BL/6J for 10 generations. These B6.129S7-Ldlrtm1Her/J (Stock No. 002207) mice were then backcrossed to AKR/J (Stock No. 000648) using a speed congenic protocol to produce this strain.
|Allele Name||targeted mutation 1, Joachim Herz|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||LDLR-; LDLR KO; LDLr0; LDLrKO; LDLr-KO; Ldlrtm1Her|
|Gene Symbol and Name||Ldlr, low density lipoprotein receptor|
|Site of Expression||Immunoblot analysis of liver membranes detected a truncated protein in homozygous mutant animals.|
|Strain of Origin||129S7/SvEvBrd-Hprt+|
|General Note||When used in bone marrow transplant into Ldlrtm1Her homozygous mice, Abca1tm1Jdm Abcg1tm1Dgen homozygous cells accelerate the development of atherosclerosis. (J:130777) |
|Molecular Note||Insertion of a neomycin resistance cassette into exon 4. The authors predict that the targeted allele would encode a truncated non-functional protein that will not bind LDL, and that lacks a membrane spanning segment. Immunoblot analysis of liver membranes detected a truncated protein in homozygous mutant animals.|
|Mutations Made By|| |
Dr. Joachim Herz, Univ of Texas Southwest Med Ctr Dallas
When maintaining a live colony, these mice can be bred as homozygotes.
When using the AK.129S7(B6)-Ldlrtm1Her/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007070 in your Materials and Methods section.