D2.129P2(B6)-Nr5a1^tm2Klp/EiJ

Stock number: 007042
Product name: SF1 floxed
Research areas: Research Tools

Description

These floxed mutant mice possess loxP sites flanking the C-terminal coding exon of the Nr5a1 gene. This strain may be useful for generating conditional mutations in applications studying steroidogenic factors and pituitary gonadotrope function.

Detailed description

Mice homozygous for this floxed allele are viable and fertile. These mutant mice have loxP sites flanking the C-terminal coding exon. When bred to Cre-recombinase expressing mice, offspring will have a deletion of this exon in the cre expressing tissue(s). These floxed mice may be useful in studying steroidogenic factors and pituitary gonadotrope function.

For example, when crossed to a strain expressing Cre recombinase in the anterior and intermediate lobes of the pituitary gland (see Stock No. 004426), this mutant mouse strain may be useful in studies of pituitary gonadotrope function.

Development

A targeting vector was designed to place a loxP site upstream, and a loxP site and neomycin resistance cassette downstream of the C-terminal coding exon of the targted gene. The construct was electroporated into 129P2/OlaHsd-derived E14TG2a embryonic stem (ES) cells. Following blastocyst injection of correctly targeted ES cells, chimeric mice transmitted this "floxed" allele to produce mutant mice. These mutant mice have been backcrossed for at least 9 generations to DBA/2J inbred mice (Stock No. 000671).

Allele

Symbol: Nr5a1^tm2Klp
Name: targeted mutation 2, Keith L Parker
MGI accession ID: MGI:2180013
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Nr5a1
Marker name: nuclear receptor subfamily 5, group A, member 1
Chromosome: 2
Strain of origin: 129P2/OlaHsd
Molecular note: A single loxP site was inserted into an intron 5' to the last coding exon, and a loxP site followed by a neomycin resistance cassette was inserted 3' to this exon. This mutation flanks sequences encoding motifs essential for transcriptional activity as well as the transcription termination and polyadenyulation sequences.