These floxed mutant mice possess loxP sites flanking the C-terminal coding exon of the Nr5a1 gene. This strain may be useful for generating conditional mutations in applications studying steroidogenic factors and pituitary gonadotrope function.
Eva Eicher, The Jackson Laboratory
Genetic Background | Generation |
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000924 C57BL/6JEiJ |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Nr5a1 | nuclear receptor subfamily 5, group A, member 1 |
Mice homozygous for this floxed allele are viable and fertile. These mutant mice have loxP sites flanking the C-terminal coding exon. When bred to Cre-recombinase expressing mice, offspring will have a deletion of this exon in the cre expressing tissue(s). These floxed mice may be useful in studying steroidogenic factors and pituitary gonadotrope function.
For example, when crossed to a strain expressing Cre recombinase in the anterior and intermediate lobes of the pituitary gland (see Stock No. 004426), this mutant mouse strain may be useful in studies of pituitary gonadotrope function.
A targeting vector was designed to place a loxP site upstream, and a loxP site and neomycin resistance cassette downstream of the C-terminal coding exon of the targted gene. The construct was electroporated into 129P2/OlaHsd-derived E14TG2a embryonic stem (ES) cells. Following blastocyst injection of correctly targeted ES cells, chimeric mice transmitted this "floxed" allele to produce mutant mice. These mutant mice have been backcrossed for at least 12 generations to C57BL/6JEiJ inbred mice (Stock No. 000924).
Allele Name | targeted mutation 2, Keith L Parker |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | F; Sf1Fl |
Gene Symbol and Name | Nr5a1, nuclear receptor subfamily 5, group A, member 1 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 2 |
Molecular Note | A single loxP site was inserted into an intron 5' to the last coding exon, and a loxP site followed by a neomycin resistance cassette was inserted 3' to this exon. This mutation flanks sequences encoding motifs essential for transcriptional activity as well as the transcription termination and polyadenyulation sequences. |
Mutations Made By | Dr. Keith Parker, Univ Texas Southwestern Medical Center |
When maintaining a live colony, homozygous mice are bred.
When using the SF1 floxed mouse strain in a publication, please cite the originating article(s) and include JAX stock #007041 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Nr5a1<tm2Klp> |
Frozen Mouse Embryo | B6Ei.129P2-Nr5a1<tm2Klp>/EiJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6Ei.129P2-Nr5a1<tm2Klp>/EiJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6Ei.129P2-Nr5a1<tm2Klp>/EiJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6Ei.129P2-Nr5a1<tm2Klp>/EiJ Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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