D2.129(B6)-Nr0b1^tm1.1Lja/EiJ

Stock number: 007007
Product name: D2-Dax1-

Development

The Nr0b1^tm1Lja floxed targeted mutation was generated in the mixed 129-derived ES cell line R1 and the resulting mice were bred with 129S1/SvImJ. This floxed allele arrived at The Jackson Laboratory on a primarily 129 background and was backcrossed once to C57BL/6J and then backcrossed repeatedly to C57BL/6JEi in the laboratory of Dr. Eva Eicher. This was then bred with C57BL/6J-Tg(Zp3-cre)93Knw to excise the floxed exon 2. The resulting Nr0b1^tm1.1Lja allele was backcrossed from a B6 background onto the DBA/2J background. On this DBA/2J background it proved possible to maintain the strain by breeding homozygous females with hemizygous males. In 2007 sperm were cryopreserved from hemizygous males at generation N10F6.

Allele

Symbol: Nr0b1^tm1.1Lja
Name: targeted mutation 1.1, J Larry Jameson
MGI accession ID: MGI:2159321
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: Ahch^delta2; Dax1-; Dax1KO
Marker symbol: Nr0b1
Marker name: nuclear receptor subfamily 0, group B, member 1
Marker synonyms: Ahc; AHCH; AHX; DAX1; DAX-1; DSS; GTD; HHG; NROB1; SRXY2
Chromosome: X
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: This allele is a derivative of Nr0b1^tm1Lja in which Cre-mediated recombination in vivo under control of a CMV promoter excised exon 2 in the germline. RT-PCR analyses of pituitary, adrenal glands, and gonads did not detect full length transcript in either homozygous female mice or hemizygous male mice.