Eva Eicher, The Jackson Laboratory
The Nr0b1tm1Lja floxed targeted mutation was generated in the mixed 129-derived ES cell line R1 and the resulting mice were bred with 129S1/SvImJ. This floxed allele arrived at The Jackson Laboratory on a primarily 129 background and was backcrossed once to C57BL/6J and then backcrossed repeatedly to C57BL/6JEi in the laboratory of Dr. Eva Eicher. This was then bred with C57BL/6J-Tg(Zp3-cre)93Knw to excise the floxed exon 2. The resulting Nr0b1tm1.1Lja allele was backcrossed from a B6 background onto the DBA/2J background. On this DBA/2J background it proved possible to maintain the strain by breeding homozygous females with hemizygous males. In 2007 sperm were cryopreserved from hemizygous males at generation N10F6.
|Allele Name||targeted mutation 1.1, J Larry Jameson|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Ahchdelta2; Dax1-; Dax1KO|
|Gene Symbol and Name||Nr0b1, nuclear receptor subfamily 0, group B, member 1|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||This allele is a derivative of Nr0b1tm1Lja in which Cre-mediated recombination in vivo under control of a CMV promoter excised exon 2 in the germline. RT-PCR analyses of pituitary, adrenal glands, and gonads did not detect full length transcript in either homozygous female mice or hemizygous male mice.|