B6Ei.129-Nr0b1^tm1Lja/EiJ

Stock number: 007006
Product name: B6Ei-Dax1^flox
Strain synonyms: 6-Dax1^flox

Development

The Nr0b1^tm1Lja floxed targeted mutation was generated in the mixed 129-derived ES cell line R1 and the resulting mice were bred with 129S1/SvImJ. This floxed allele arrived at The Jackson Laboratory on a primarily 129 background and was backcrossed once to C57BL/6J and then backcrossed repeatedly to C57BL/6JEi in the laboratory of Dr. Eva Eicher. In 2007 sperm was cryopreserved from hemizygous males at generation N10F6. Cryo-recovery will use C57BL/6J oocytes unless specified otherwise.

Allele

Symbol: Nr0b1^tm1Lja
Name: targeted mutation 1, J Larry Jameson
MGI accession ID: MGI:2159305
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: Ahch^flox2; B6-DAX1; Dax1^flox
Marker symbol: Nr0b1
Marker name: nuclear receptor subfamily 0, group B, member 1
Marker synonyms: Ahc; AHCH; AHX; DAX1; DAX-1; DSS; GTD; HHG; NROB1; SRXY2
Chromosome: X
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A floxed PGK-neomycin resistance cassette was inserted downstream of exon 2 and a third loxP site was inserted upstream of the splice acceptor signal in exon 2.