Because the naked cuticle (NKD) genes are considered targets for Wnt/β-catenin signaling, these nkd1lacZ mutant mice (as well as the similar nkd2lacZ mutant strain; Stock No. 006960) may be useful as a reporter for Wnt/β-catenin-dependent transcriptional activity during embryonic development or in adult mice.
Keith Wharton, UT Southwestern Medical Center
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Reporter, Null/Knockout) | Nkd1 | naked cuticle 1 |
Mice homozygous for this nkd1lacZ mutant allele are viable and fertile with slightly reduced mean litter sizes. Northern blot of lung tissue, as well as western blot of embryonic tissue, confirms that the deleted exons (encoding the Dvl-binding domain) are not expressed in homozygous mutants. However, the endogenous promoter directs expression of the internal ribosome entry site-β-galactosidase (IRES-lacZ) fusion gene in a manner that closely mimics the patterns of the endogenous gene mRNAs. Because the naked cuticle (NKD) genes are considered targets for Wnt/β-catenin signaling, these nkd1lacZ mutant mice (as well as the similar nkd2lacZ mutant strain; Stock No. 006960) may be useful as a reporter for Wnt/β-catenin-dependent transcriptional activity during embryonic development or in adult mice.
A targeting vector was designed to insert a stop codon into exon 5 at amino acid 91, as well as replace exons 5-10 of the targeted gene with an internal ribosome entry site-β-galactosidase (IRES-lacZ) fusion gene and neomycin cassette. The construct was electroporated into 129SvEv-derived embryonic stem (ES) cells. Correctly targeted ES cells were microinjected into blastocysts, and chimeric offspring were bred to C57BL/6 mice. Heterozygous mutants were bred to generate homozygotes, which were bred together for many generations prior to arrival at The Jackson Laboratory.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
---|---|
Site of Expression | lacZ expression closely mimics the patterns of the endogenous gene. |
Allele Name | targeted mutation 1, Keith Wharton |
---|---|
Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | nkd1lacZ |
Gene Symbol and Name | Nkd1, naked cuticle 1 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | lacZ expression closely mimics the patterns of the endogenous gene. |
Strain of Origin | 129/SvEv |
Chromosome | 8 |
Molecular Note | A targeting vector was designed to insert a stop codon into exon 5 at amino acid 91, as well as replace exons 5-10 of the targeted gene with an internal ribosome entry site-b-galactosidase (IRES-lacZ) fusion gene and neomycin cassette. The construct was electroporated into 129SvEv-derived embryonic stem (ES) cells. Northern blot analysis of lung tissue and Western blot analysis of embronic tissue in homozygotes confirm lack of normal mRNA expression, while X-gal staining closely mimics dynamic expression pattern of normal Nkd1 mRNA. |
Mutations Made By | Keith Wharton, UT Southwestern Medical Center |
When maintaining a live colony, homozygous mice are bred together.
When using the B6;129S-Nkd1tm1Kwha/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #006958 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Nkd1<tm1Kwha> |
Frozen Mouse Embryo | B6;129S-Nkd1<tm1Kwha>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S-Nkd1<tm1Kwha>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S-Nkd1<tm1Kwha>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129S-Nkd1<tm1Kwha>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.