These floxed mutant mice possess loxP sites flanking exons 4-5 of the Spi1 gene. This strain may be useful for generating conditional mutations in studies relating to cancer and hematopoiesis.
Daniel G. Tenen, Beth Israel Deaconess Medical Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Spi1 | spleen focus forming virus (SFFV) proviral integration oncogene |
NOTE: Despite these mice being backcrossed onto the C57BL/6 genetic background, occasional albino pups may be observed. The donating investigator confirms this observation and suggests the targeted mutation may have an as of yet uncharacterized effect upon coat color genes.
Mice that are homozygous for this "PU.1F" conditional allele are viable and fertile. When PU.1F mice are bred to mice expressing Cre recombinase, exons 4-5 of the targeted gene are deleted in the cre-expressing tissues in the offspring. These mice may be useful in studying T cell lymphoma, AML and other cancers, as well as transcription factors, hematopoiesis, and the development of multiple cell lineages.
For example, when PU.1F mice are crossed with mice expressing the interferon- or dsRNA-inducible Mx1-cre transgenic mice (see Stock No. 003556), this mutant mouse strain may be useful in studies of hematopoietic stem cells.
NOTE: Despite these mice being backcrossed onto the C57BL/6 genetic background, occasional albino pups may be observed. The donating investigator confirms this observation and suggests the targeted mutation may have an as of yet uncharacterized effect upon coat color genes.
A targeting vector was designed to insert loxp sites flanking exons 4-5 of the endogenous mouse PU.1 (or Sfpi1) gene, as well as a loxp-flanked PGK-neo cassette downstream of exon 5. This construct was electroporated into 129-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts, and the resulting chimeric males were bred to C57BL/6 females. These PU.1F mice were then backcrossed to C57BL/6 for at least 5 generations. At some point, these mice were bred with Mx1-Cre transgenic mice (congenic (N9) on a C57BL/6 background) and double mutant mice were subsequently sent to The Jackson Laboratory. Upon arrival, mice were selected for the PU.1F targeted mutation and (against the Mx1-Cre transgene) to establish this strain.
Allele Name | targeted mutation 2, Daniel G Tenen |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | PU.1F |
Gene Symbol and Name | Spi1, spleen focus forming virus (SFFV) proviral integration oncogene |
Gene Synonym(s) | |
Strain of Origin | Not Specified |
Chromosome | 2 |
Molecular Note | LoxP sites were inserted in intron 3 and a loxP-flanked neomycin selection cassette was inserted into intron 5. |
Mutations Made By | Daniel Tenen, Beth Israel Deaconess Medical Center |
When maintaining a live colony, homozygous mice are bred.
NOTE: Despite these mice being backcrossed onto the C57BL/6 genetic background, occasional albino pups may be observed. The donating investigator confirms this observation and suggests the targeted mutation may have an as of yet uncharacterized effect upon coat color genes.
When using the PU.1F mouse strain in a publication, please cite the originating article(s) and include JAX stock #006922 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Spi1<tm2Dgt> |
Frozen Mouse Embryo | B6.Cg-Spi1<tm2Dgt>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Spi1<tm2Dgt>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Spi1<tm2Dgt>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Spi1<tm2Dgt>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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