B6.Cg-Spi1^tm2Dgt/J

Stock number: 006922
Product name: PU.1^F
Research areas: Cancer Research, Developmental Biology Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools

Description

These floxed mutant mice possess loxP sites flanking exons 4-5 of the Spi1 gene. This strain may be useful for generating conditional mutations in studies relating to cancer and hematopoiesis.

Detailed description

Mice that are homozygous for this "PU.1F" conditional allele are viable and fertile. When PU.1^F mice are bred to mice expressing Cre recombinase, exons 4-5 of the targeted gene are deleted in the cre-expressing tissues in the offspring. These mice may be useful in studying T cell lymphoma, AML and other cancers, as well as transcription factors, hematopoiesis, and the development of multiple cell lineages.

For example, when PU.1^F mice are crossed with mice expressing the interferon- or dsRNA-inducible Mx1-cre transgenic mice (see Stock No. 003556), this mutant mouse strain may be useful in studies of hematopoietic stem cells.

NOTE: Despite these mice being backcrossed onto the C57BL/6 genetic background, occasional albino pups may be observed. The donating investigator confirms this observation and suggests the targeted mutation may have an as of yet uncharacterized effect upon coat color genes.

Development

A targeting vector was designed to insert loxp sites flanking exons 4-5 of the endogenous mouse PU.1 (or Sfpi1) gene, as well as a loxp-flanked PGK-neo cassette downstream of exon 5. This construct was electroporated into 129-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts, and the resulting chimeric males were bred to C57BL/6 females. These PU.1^F mice were then backcrossed to C57BL/6 for at least 5 generations. At some point, these mice were bred with Mx1-Cre transgenic mice (congenic (N9) on a C57BL/6 background) and double mutant mice were subsequently sent to The Jackson Laboratory. Upon arrival, mice were selected for the PU.1^F targeted mutation and (against the Mx1-Cre transgene) to establish this strain.

Allele

Symbol: Spi1^tm2Dgt
Name: targeted mutation 2, Daniel G Tenen
MGI accession ID: MGI:3688438
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Spi1
Marker name: spleen focus forming virus (SFFV) proviral integration oncogene
Chromosome: 2
Strain of origin: Not Specified
Molecular note: LoxP sites were inserted in intron 3 and a loxP-flanked neomycin selection cassette was inserted into intron 5.