Estimated Removal of Live Colony date: 20 June 2019.
These inhibitor of kappaB kinase epsilon knock-out mutant mice may be useful in immunological studies involving IFN signaling and host responses to infection.
Tom Maniatis, Columbia University College of Physicians and Surgeons
Homozygous IKKepsilon mutant mice are viable and fertile. Homozygous deletion results in a complete loss of the endogenous kinase function in lung, spleen, and embryonic fibroblasts. These mice have increased susceptibility to viral infection due to defective inteferon (IFN) signaling. These IKKepsilon mutant mice may be useful in immunological studies involving IFN signaling and host responses to infection.
The donating investigator reports that a targeting vector was used to replace exons 4-6 of the targeted gene with a PGK-neo cassette. The construct was electroporated into "B6/S129"-hybrid derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts, and chimeric offspring were bred to C57BL/6J mice. Heterozygous mutants were bred to inbred C57BL/6J mice for at least 5 generations prior to arrival at The Jackson Laboratory.
A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. One of the 43 markers, on Chromosome 3, was segregating.
|Allele Name||targeted mutation 1, Tom Maniatis|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Ikbketm1Tman; targeted mutation 1, Tom Maniatis|
|Gene Symbol and Name||Ikbke, inhibitor of kappaB kinase epsilon|
|Gene Synonym(s)||expressed sequence AW558201; AW558201; IKK-E; IKK-i; IKKE; IKKepsilon; IKKI|
|Strain of Origin||129 and C57BL/6|
|Molecular Note||Exons 4-6 of the targeted gene were replaced with a PGK-neo cassette. Mice show a complete loss of the endogenous kinase function in lung, spleen, and embryonic fibroblasts.|
|Mutations Made By|| |
Tom Maniatis, Columbia University College of Physicians and Surgeons
When maintaining a live colony, homozygous mice may be bred together.
When using the B6.Cg-Ikbketm1Tman/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #006908 in your Materials and Methods section.
|Heterozygous for Ikbke<tm1Tman>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
|Frozen Mouse Embryo||$2,595.00 per straw or vial|
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