B6.129-Galnt13^tm1Jxm/J

Stock number: 006896
Research areas: Research Tools

Description

Mice homozygous for the floxed targeted mutation of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 13 gene are viable, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exons are deleted by cre expression to produce a null allele. This glycosyltransferase is also sometimes termed as ppGalNAcT-13 in studies of human tissues.

Detailed description

Mice homozygous for the floxed targeted mutation are viable, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exons are deleted by cre expression to produce a null allele. This glycosyltransferase is also sometimes termed as ppGalNAcT-13 in studies of human tissues.

Development

A targeting vector was used to flank a single exon encoding almost 61 amino acids of the catalytic domain with loxP sites and place a neomycin resistance gene in the downstream intron. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were backcrossed to C57BL/6 ten times by the donating laboratory. The neomycin resistance cassette does not affect expression levels of the targeted gene.

Allele

Symbol: Galnt13^tm1Jxm
Name: targeted mutation 1, Jamey Marth
MGI accession ID: MGI:3699607
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: Glc-NAc-T13
Marker symbol: Galnt13
Marker name: polypeptide N-acetylgalactosaminyltransferase 13
Marker synonyms: pp-GalNAc-T13; T13
Chromosome: 2
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A targeting vector was used to flank an exon encoding almost 61 amino acids of the catalytic domain with loxP sites and place a neomycin resistance gene in the downstream intron.
General note: According to the originating lab, this allele is the one created in J:67674, not B4galnt13. The gene was misidentified in the publication, and Galnt13 mutant mice were generated.