Cre recombinase expression in these mice is controlled by the zona pellucida 3 (Zp3) gene and is expressed in oocytes.
Jamey D Marth, Burnham Inst at Univ Calif Santa Barbara
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
This is a transgenic line in which Cre recombinase expression is controlled by the regulatory sequences from the mouse zona pellucida 3 (Zp3) gene, which is normally expressed exclusively in the growing oocyte prior to the completion of the first meiotic division. This strain is useful for deleting a loxP-flanked genomic segment during oogenesis and thereby producing embryos and offspring that bear the recombined and deleted genomic element(s).
The ZP3-Cre transgene was constructed by linking the Zp3 promoter to the Cre gene as well as the human growth hormone splicing and polyadenylation signals.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | cre recombinase is expressed in oocytes. |
Allele Name | transgene insertion 1, Gerald W Hart |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | ZP3Cre; ZP3-Cre |
Gene Symbol and Name | Tg(Zp3-cre)1Gwh, transgene insertion 1, Gerald W Hart |
Gene Synonym(s) | |
Promoter | Zp3, zona pellucida glycoprotein 3, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | cre recombinase is expressed in oocytes. |
Strain of Origin | Not Specified |
Chromosome | UN |
Molecular Note | A 6 kb fragment encompassing the promoter sequence of Zp3 was adjoined to the cre recombinase coding region and human growth hormone splicing and polyadenylation signals. The Zp3 promoter region was reported to direct expression in oocytes. |
Mutations Made By | Jamey Marth, Burnham Inst at Univ Calif Santa Barbara |
When maintained as a live colony, hemizygote x C57BL/6 crosses are typically used.
When using the ZP3-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #006888 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous for Tg(Zp3-cre)1Gwh |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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