B6.Cg-Tg(Zp3-cre)1Gwh/J

Stock number: 006888
Product name: ZP3-Cre
Research areas: Research Tools

Description

Cre recombinase expression in these mice is controlled by the zona pellucida 3 (Zp3) gene and is expressed in oocytes.

Detailed description

This is a transgenic line in which Cre recombinase expression is controlled by the regulatory sequences from the mouse zona pellucida 3 (Zp3) gene, which is normally expressed exclusively in the growing oocyte prior to the completion of the first meiotic division. This strain is useful for deleting a loxP-flanked genomic segment during oogenesis and thereby producing embryos and offspring that bear the recombined and deleted genomic element(s).

Development

The ZP3-Cre transgene was constructed by linking the Zp3 promoter to the Cre gene as well as the human growth hormone splicing and polyadenylation signals.

Allele

Symbol: Tg(Zp3-cre)1Gwh
Name: transgene insertion 1, Gerald W Hart
MGI accession ID: MGI:2387870
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Zp3-cre)1Gwh
Marker name: transgene insertion 1, Gerald W Hart
Chromosome: UN
Strain of origin: Not Specified
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: cre recombinase is expressed in oocytes.
Molecular note: A 6 kb fragment encompassing the promoter sequence of Zp3 was adjoined to the cre recombinase coding region and human growth hormone splicing and polyadenylation signals. The Zp3 promoter region was reported to direct expression in oocytes.