These SM22-rtTA mice provide a Tet-On tool that allows the inducible expression of genes in smooth muscle cells.
Dr. James West, Univ. of Colorado Health Sciences Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Transactivator) |
Transgenic SM22-rtTA mice are viable and fertile. These mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the murine SM22-alpha (SM22α or transgelin) promoter. When hemizygotes are mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene in the bitransgenic offspring is inducible in smooth muscle cells with administration of the tetracycline analog, doxycycline. These SM22-rtTA mice provide a "Tet-On" tool that allows the inducible expression of genes in smooth muscle cells.
The SM22-rtTA transgene contains a 488-bp fragment of the murine smooth muscle-specific SM22-alpha (or transgelin) promoter that drives expression of the reverse tetracycline transactivator gene (rtTA). The transgene was injected into FVB/N embryos. Transgenic mice (founder line E) on the FVB/N genetic background were bred together for many generations prior to arrival at The Jackson Laboratory.
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
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Site of Expression | Expresses rtTA in vascular smooth muscle. |
Allele Name | transgene insertion E1, James West |
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Allele Type | Transgenic (Transactivator) |
Allele Synonym(s) | SM22-rtTA |
Gene Symbol and Name | Tg(Tagln-rtTA)E1Jwst, transgene insertion E1, James West |
Gene Synonym(s) | |
Promoter | Tagln, transgelin, mouse, laboratory |
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
Site of Expression | Expresses rtTA in vascular smooth muscle. |
Strain of Origin | FVB/N |
Chromosome | UN |
Molecular Note | The transgene has the 488-bp fragment of the murine smooth muscle-specific SM22-alpha (Tagln or transgelin) promoter to drive expression of the reverse tetracycline transactivator gene (rtTA). |
Mutations Made By | Dr. James West, Univ. of Colorado Health Sciences Center |
When maintaining a live colony, homozygous mice may be bred together.
When using the FVB/N-Tg(Tagln-rtTA)E1Jwst/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #006875 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizyous or Non Carrir for Tg(Tagln-rtTA)E1Jwst |
Frozen Mouse Embryo | FVB/N-Tg(Tagln-rtTA)E1Jwst/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tg(Tagln-rtTA)E1Jwst/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tg(Tagln-rtTA)E1Jwst/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | FVB/N-Tg(Tagln-rtTA)E1Jwst/J Frozen Embryo | $3373.50 |
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