FVB/N-Tg(Tagln-rtTA)E1Jwst/J

Stock number: 006875
Research areas: Internal/Organ Research, Research Tools

Description

These SM22-rtTA mice provide a Tet-On tool that allows the inducible expression of genes in smooth muscle cells.

Detailed description

Transgenic SM22-rtTA mice are viable and fertile. These mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the murine SM22-alpha (SM22α or transgelin) promoter. When hemizygotes are mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene in the bitransgenic offspring is inducible in smooth muscle cells with administration of the tetracycline analog, doxycycline. These SM22-rtTA mice provide a "Tet-On" tool that allows the inducible expression of genes in smooth muscle cells.

Development

The SM22-rtTA transgene contains a 488-bp fragment of the murine smooth muscle-specific SM22-alpha (or transgelin) promoter that drives expression of the reverse tetracycline transactivator gene (rtTA). The transgene was injected into FVB/N embryos. Transgenic mice (founder line E) on the FVB/N genetic background were bred together for many generations prior to arrival at The Jackson Laboratory.

Allele

Symbol: Tg(Tagln-rtTA)E1Jwst
Name: transgene insertion E1, James West
MGI accession ID: MGI:3703755
Generation method: Transgenic
Attributes: Transactivator
Marker symbol: Tg(Tagln-rtTA)E1Jwst
Marker name: transgene insertion E1, James West
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: rtTA,reverse tetracycline-controlled transactivator,E. coli
Site of expression: Expresses rtTA in vascular smooth muscle.
Molecular note: The transgene has the 488-bp fragment of the murine smooth muscle-specific SM22-alpha (Tagln or transgelin) promoter to drive expression of the reverse tetracycline transactivator gene (rtTA).