These floxed mutant mice possess loxP sites flanking exon 3 of the Gabra4 gene. This strain may be useful for generating conditional mutations in applications related to neurological studies.
Gregg E Homanics, University of Pittsburgh
Mice homozygous for this GABAA-R alpha4F allele are viable and fertile. These mutant mice have loxP sites flanking exon 3 of the targeted gene. When bred to Cre-recombinase expressing mice, offspring will have a deletion of exon 3 in the cre expressing tissue(s). These "floxed" mice may be useful in neurological studies including behavior and neurotransmitter function.
Of note, several strains bearing gamma-aminobutyric acid (GABA-A) receptor mutations are available from this donating investigator (Dr. Gregg Homanics, University of Pittsburgh), including Gabra1 (Stock No. 004318), Gabra4 (Stock No. 006874), Gabra6 (Stock No. 002710), Gabrb3 (Stock No. 002711), Gabrd (Stock No. 003725), and Gabrg2 (Stock No. 003137).
A targeting vector was designed to place a loxP site upstream and a frt-flanked neomycin resistance gene and loxP site downstream of exon 3 of the targeted gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were microinjected into C57BL/6J blastocysts. Chimeric mice were bred to C57BL/6J females. Offspring with the "floxed" exon 3 allele and neomycin cassette (Fneo) were bred to actin-FLPe mice (on a mixed C57BL/6J and SJL genetic background). Offspring with "floxed" exon 3 and excised neo allele (F) were subsequently backcrossed to C57BL/6J for 2 generations. Mice were then backcrossed to an EIIa-Cre transgenic mouse strain (C57BL/6J congenic background, see Stock No. 003724). The resulting mice still containing the non-recombined "floxed" exon 3 (F) allele that were negative for the cre transgene were bred to C57BL/6J for two more generations prior to arrival at The Jackson Laboratory. Upon arrival, mutant mice were bred to C57BL/6J for at least three generations.
|Allele Name||targeted mutation 1.2, Gregg E Homanics|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||F; GABAA-R alpha4F; Gabra4F|
|Gene Symbol and Name||Gabra4, gamma-aminobutyric acid (GABA) A receptor, subunit alpha 4|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||Offspring with a floxed exon 3 allele and neomycin cassette (Fneo) were bred to actin-FLPe mice to remove the Neomycin cassette.|
|Mutations Made By|| |
Gregg Homanics, University of Pittsburgh
When maintaining a live colony, homozygous mice can be bred.
When using the GABAA-R α4F mouse strain in a publication, please cite the originating article(s) and include JAX stock #006874 in your Materials and Methods section.