B6.129-Gabra4^tm1.2Geh/J

Stock number: 006874
Product name: GABA_A-R α4^F
Research areas: Neurobiology Research, Research Tools

Description

These floxed mutant mice possess loxP sites flanking exon 3 of the Gabra4 gene. This strain may be useful for generating conditional mutations in applications related to neurological studies.

Detailed description

Mice homozygous for this GABA_A-R alpha4^F allele are viable and fertile. These mutant mice have loxP sites flanking exon 3 of the targeted gene. When bred to Cre-recombinase expressing mice, offspring will have a deletion of exon 3 in the cre expressing tissue(s). These "floxed" mice may be useful in neurological studies including behavior and neurotransmitter function.

Of note, several strains bearing gamma-aminobutyric acid (GABA-A) receptor mutations are available from this donating investigator (Dr. Gregg Homanics, University of Pittsburgh), including Gabra1 (Stock No. 004318), Gabra4 (Stock No. 006874), Gabra6 (Stock No. 002710), Gabrb3 (Stock No. 002711), Gabrd (Stock No. 003725), and Gabrg2 (Stock No. 003137).

Development

A targeting vector was designed to place a loxP site upstream and a frt-flanked neomycin resistance gene and loxP site downstream of exon 3 of the targeted gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were microinjected into C57BL/6J blastocysts. Chimeric mice were bred to C57BL/6J females. Offspring with the "floxed" exon 3 allele and neomycin cassette (Fneo) were bred to actin-FLPe mice (on a mixed C57BL/6J and SJL genetic background). Offspring with "floxed" exon 3 and excised neo allele (F) were subsequently backcrossed to C57BL/6J for 2 generations. Mice were then backcrossed to an EIIa-Cre transgenic mouse strain (C57BL/6J congenic background, see Stock No. 003724). The resulting mice still containing the non-recombined "floxed" exon 3 (F) allele that were negative for the cre transgene were bred to C57BL/6J for two more generations prior to arrival at The Jackson Laboratory. Upon arrival, mutant mice were bred to C57BL/6J for at least three generations.

Allele

Symbol: Gabra4^tm1.2Geh
Name: targeted mutation 1.2, Gregg E Homanics
MGI accession ID: MGI:3704092
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Gabra4
Marker name: gamma-aminobutyric acid (GABA) A receptor, subunit alpha 4
Chromosome: 5
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: Offspring with a floxed exon 3 allele and neomycin cassette (Fneo) were bred to actin-FLPe mice to remove the Neomycin cassette.