B6.Cg-Tg(Ins1-DsRed*T4)32Hara/J

Stock number: 006872
Research areas: Research Tools

Description

This strain has been discontinued, please see Stock No. 006866 STOCK Tg(Ins1-DsRed*T4)32Hara/J

Detailed description

Mice hemizygous for this "MIP-RFP" transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the DsRed.T4 variant of Enhanced Red Fluorescent Protein under the control of mouse insulin 1 promoter. Fluorescence is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adult. Transgenic mice exhibit normal glucose tolerance and pancreatic insulin levels. While the human growth hormone sequence in the transgene is designed to enhance expression of the fluorescent protein and is not expressed in a similarly constructed transgene (see Stock No. 006864), whether or not it is expressed for this strain was not characterized. This mutant mouse strain may be useful in studies of pancreatic development and pancreatic beta islet cell biology.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A transgenic construct containing the Red Fluorescent Protein (DsRed.T4 variant) gene and 2.1kb of sequence from the human growth hormone gene (to confer high expression of the DsRed.T4) under the control of the mouse insulin 1 promoter was injected into fertilized CD1 mouse eggs. Founder line 32 was subsequently established. Upon arrival at The Jackson Laboratory, transgenic mice on this outbred background (see Stock No. 006866) were bred to C57BL/6J inbred mice for at least 5 generations to generate this congenic strain (Stock No. 006872).

Allele

Symbol: Tg(Ins1-DsRed*T4)32Hara
Name: transgene insertion 32, Manami Hara
MGI accession ID: MGI:3704922
Generation method: Transgenic
Attributes: Reporter
Synonyms: MIP-RFP (line 12); MIP-RFP (line 32)
Marker symbol: Tg(Ins1-DsRed*T4)32Hara
Marker name: transgene insertion 32, Manami Hara
Marker synonyms: MIP-RFP (line 12); MIP-RFP (line 32)
Chromosome: UN
Strain of origin: CD-1
Expressed genes: RFP,Red Fluorescent Protein,coral
Site of expression: DsRed.T4 expression is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adulthood.
Molecular note: The transgenic construct consists of the Red Fluorescent Protein (DsRed.T4 variant) gene and 2.1kb of sequence from the human growth hormone gene (to confer high expression of the DsRed.T4) under the control of the mouse insulin 1 promoter. Line 32 was subsequently established. Fluorescence is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adult.