B6.129S6-Per2^tm1Jt/J

Stock number: 006852
Product name: mPer2^Luc
Research areas: Neurobiology Research, Research Tools

Description

These targeted reporter mutant mice express PER2::LUC bioluminescence similar to endogenous Per2 expression, and may be useful as a real-time reporter of circadian dynamics in different tissues.

Detailed description

Mice homozygous for this "mPer2^Luc" mutation are viable and fertile with no developmental or morphological differences compared to wildtype littermates. Expression of PERIOD2::LUCIFERASE (or mPER2::LUC) fusion protein during peak periods is similar to endogenous pPER2 patterns. Homozygous mice have normal entrainment and circadian behaviors. These mPer2^Luc knock-in mice may be useful as a real-time reporter of circadian dynamics in different tissues.

Development

A targeting vector was designed to insert the firefly luciferase (luc) gene followed by an in-frame loxP-flanked neomycin resistance gene between exon 23 and the 3' UTR of the period circadian clock 2 (Per2) gene. The construct was electroporated into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. Chimeric males were bred to C57BL/6J females. Heterozygotes were backcrossed to C57BL/6J inbred mice for 7-11 generations. Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Per2^tm1Jt
Name: targeted mutation 1, Joseph S Takahashi
MGI accession ID: MGI:3040876
Generation method: Targeted
Attributes: Reporter; No functional change
Marker symbol: Per2
Marker name: period circadian clock 2
Chromosome: 1
Strain of origin: 129S6/SvEvTac
Expressed genes: luc,luciferase,firefly
Site of expression: Circadian patterns of Per2-Luc fusion protein expression is seen in the following peripheral tissues: SCN, cornea, liver, pituitary, kidney, RCA, lung and tail).
Molecular note: A luciferase gene and floxed neo cassette were inserted between exon 23 and the 3' UTR. Mutant transcript was detected in Northern blot of mutant brains and the Per2-Luc fusion protein was detected in Western blots of mutant kidneys.