B6.129S2(C)-Cxcr2^tm1Mwm/J

Stock number: 006848
Product name: mIL-8Rh-
Research areas: Cancer Research, Cell Biology Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Neurobiology Research, Research Tools

Description

Mice homozygous for this targeted mutation have several abnormalities, including neurological defects, impaired wound healing, impaired angiogenesis, altered growth of induced/implanted tumors, splenomegaly, lymphadenopathy, and increased susceptibility to various pathogens due to impaired neutrophil recruitment and decreased pathogen clearance during innate immune responses. These mutant mice may be useful in studies of inflammation and immunology and cancer biology.

Detailed description

The donating investigator reports that homozygous mice are viable and fertile but few pups are produced, and they need a gnotobiotic facility to thrive. Homozygous mice have several abnormalities, including neurological defects, impaired wound healing, impaired angiogenesis, and altered growth of induced/implanted tumors. Homozygotes may also exhibit splenomegaly, lymphadenopathy, and increased susceptibility to various pathogens due to impaired neutrophil recruitment and decreased pathogen clearance during innate immune responses. These mutant mice may be useful in studies of inflammation and immunology and cancer biology.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A targeting vector was designed to replace the single exon of the targeted gene with a PGK-neomycin resistance cassette. The construct was electroporated into the 129S2/SvPas-derived D3 embryonic stem (ES) cells, and correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric males were bred to C57BL/6J females to establish a mutant colony, and then backcrossed to BALB/c for many generations in the laboratory of Dr. Mark Moore at Genentech, Inc. Mice on this BALB/c congenic background (see Stock No. 002724) were sent to Dr. Robert Terkeltaub (Univ. of California, San Diego) where they were backcrossed for approximately 12 generations to C57BL/6, and the strain was next sent to Dr. Ann Richmond (Vanderbilt University Medical Center), who provided the strain to The Jackson Laboratory.

Allele

Symbol: Cxcr2^tm1Mwm
Name: targeted mutation 1, Mark Moore
MGI accession ID: MGI:1857153
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Cxcr2
Marker name: chemokine (C-X-C motif) receptor 2
Chromosome: 1
Strain of origin: 129S2/SvPas
Molecular note: A neomycin selection cassette replaced the entire coding sequence of the gene.