Mice homozygous for this targeted mutation have several abnormalities, including neurological defects, impaired wound healing, impaired angiogenesis, altered growth of induced/implanted tumors, splenomegaly, lymphadenopathy, and increased susceptibility to various pathogens due to impaired neutrophil recruitment and decreased pathogen clearance during innate immune responses. These mutant mice may be useful in studies of inflammation and immunology and cancer biology.
Ann Richmond, Vanderbilt University School of Medicine
The donating investigator reports that homozygous mice are viable and fertile but few pups are produced, and they need a gnotobiotic facility to thrive. Homozygous mice have several abnormalities, including neurological defects, impaired wound healing, impaired angiogenesis, and altered growth of induced/implanted tumors. Homozygotes may also exhibit splenomegaly, lymphadenopathy, and increased susceptibility to various pathogens due to impaired neutrophil recruitment and decreased pathogen clearance during innate immune responses. These mutant mice may be useful in studies of inflammation and immunology and cancer biology.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector was designed to replace the single exon of the targeted gene with a PGK-neomycin resistance cassette. The construct was electroporated into the 129S2/SvPas-derived D3 embryonic stem (ES) cells, and correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric males were bred to C57BL/6J females to establish a mutant colony, and then backcrossed to BALB/c for many generations in the laboratory of Dr. Mark Moore at Genentech, Inc. Mice on this BALB/c congenic background (see Stock No. 002724) were sent to Dr. Robert Terkeltaub (Univ. of California, San Diego) where they were backcrossed for approximately 12 generations to C57BL/6, and thestrain was next sent to Dr. Ann Richmond (Vanderbilt University Medical Center), who provided the strain to The Jackson Laboratory.
|Allele Name||targeted mutation 1, Mark Moore|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||CXCR2 -; CXCR2-; CXCR-; Cmkar2tm1Mwm; Cmkartm1Mwm; Il8rbtm1Mwm; mCXCR2-; mCXCR-; mIL-8Rh KO; mIL-8Rh-|
|Gene Symbol and Name||Cxcr2, chemokine (C-X-C motif) receptor 2|
|Gene Synonym(s)||interleukin 8 receptor, beta; CD128; CD182; CDw128b; CMKAR2; Cmkar2; Cmkar2; G-protein coupled receptor 16; Gpcr16; Gpcr16; IL-8Rh; IL-8rb; IL8R2; IL8RA; IL8RB; Il8rb; Il8rb; chemokine (C-X-C) receptor 2; interleukin 8 receptor, beta|
|Strain of Origin||129S2/SvPas|
|Molecular Note||A neomycin selection cassette replaced the entire coding sequence of the gene.|
|Mutations Made By|| |
Dr. Mark Moore, Deltagen
When maintaining a live colony, these mice are bred as heterozyotes. The donating investigator breeds heterozygotes and wildtype siblings with the best results obtained in a germ free gnotobiotic environment.
|Please inquire about possible genotypes.|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in
order to provide the minimum number of animals, animals will ship within 25 weeks.
The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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