These floxed mutant mice possess loxP sites flanking exon 2 of the Dag1 gene. This strain may be useful for generating conditional mutations in applications related to the nervous system and muscular dystrophies.
Kevin Campbell, University of Iowa
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Dag1 | dystroglycan 1 |
Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express cre recombinase, resulting offspring can have exon 2 of the targeted gene deleted in the cre-expressing tissue(s). As the targeted gene has three loxP sites, other genotypes may also result. These mutant mice may be useful in studying muscle disease and regeneration.
When bred to a strain expressing Cre recombinase under the control of the human glial fibrillary acidic protein promoter (GFAP) (see Stock No. 004600 for example), this mutant mouse strain may be useful in studies of brain abnormalities observed in congenital muscular dystrophies.
When bred to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771 for example), this mutant mouse strain may be useful in studies of retinal physiology.
When bred to mice carrying Tg(Mpz-cre)26Mes (see Stock No. 017927 for example) Cre recombinase expression in Schwann cells results in abnormal folding or the myelin sheath and demyelination.
A targeting vector was used to introduce a loxP site downstream of exon 2 and insert a floxed neo cassette in the upstream intron. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. ES cells were injected into blastocysts. Chimeric animals were backcrossed to 129S1/SvImJ by the donating laboratory for six generations.
Allele Name | targeted mutation 2, Kevin P Campbell |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Dag1flox; Dag1L; Dag1lox; DG (lox); DGlox; DG-flox |
Gene Symbol and Name | Dag1, dystroglycan 1 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 9 |
Molecular Note | Exon 2 was floxed by introducing a loxP site downstream of exon 2 and by inserting a floxed neo cassette into the upstream intron via homologous recombination. |
Mutations Made By | Kevin Campbell, University of Iowa |
When maintaining a live colony, homozygous mice may be bred.
When using the 129-Dag1tm2Kcam/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #006835 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Dag1<tm2Kcam> |
Frozen Mouse Embryo | 129-Dag1<tm2Kcam>/J | $2595.00 |
Frozen Mouse Embryo | 129-Dag1<tm2Kcam>/J | $2595.00 |
Frozen Mouse Embryo | 129-Dag1<tm2Kcam>/J | $3373.50 |
Frozen Mouse Embryo | 129-Dag1<tm2Kcam>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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