129-Dag1^tm2Kcam/J

Stock number: 006835
Strain synonyms: T30 x 129, DG-flox
Research areas: Research Tools

Description

These floxed mutant mice possess loxP sites flanking exon 2 of the Dag1 gene. This strain may be useful for generating conditional mutations in applications related to the nervous system and muscular dystrophies.

Detailed description

Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express cre recombinase, resulting offspring can have exon 2 of the targeted gene deleted in the cre-expressing tissue(s). As the targeted gene has three loxP sites, other genotypes may also result. These mutant mice may be useful in studying muscle disease and regeneration.

When bred to a strain expressing Cre recombinase under the control of the human glial fibrillary acidic protein promoter (GFAP) (see Stock No. 004600 for example), this mutant mouse strain may be useful in studies of brain abnormalities observed in congenital muscular dystrophies.

When bred to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771 for example), this mutant mouse strain may be useful in studies of retinal physiology.

When bred to mice carrying Tg(Mpz-cre)26Mes (see Stock No. 017927 for example) Cre recombinase expression in Schwann cells results in abnormal folding or the myelin sheath and demyelination.

Development

A targeting vector was used to introduce a loxP site downstream of exon 2 and insert a floxed neo cassette in the upstream intron. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 embryonic stem (ES) cells. ES cells were injected into blastocysts. Chimeric animals were backcrossed to 129S1/SvImJ by the donating laboratory for six generations.

Allele

Symbol: Dag1^tm2Kcam
Name: targeted mutation 2, Kevin P Campbell
MGI accession ID: MGI:2387358
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Dag1
Marker name: dystroglycan 1
Chromosome: 9
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: Exon 2 was floxed by introducing a loxP site downstream of exon 2 and by inserting a floxed neo cassette into the upstream intron via homologous recombination.