Mice homozygous for this targeted mutation are viable and do not display any gross physical or behavioral abnormalities. Male homozygotes are infertile due to a defect in sperm motility. Their sperm fails to develop the hyperactive motility pattern required for fertilization. No morphological differences between wildtype and mutant spermatozoa have been observed. No protein product from the targeted gene is detected in testis tissue. Female homozygotes reproduce normally. This strain may be useful in studies of male infertility.
A targeting vector containing a neomycin resistance gene was used to replace exons 5-8. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with 129S1/Sv. The strain was backcrossed three times to 129S1/Sv by the donating laboratory.
|Allele Name||targeted mutation 1, David E Clapham|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Catsper4, cation channel, sperm associated 4|
|Strain of Origin||129S4/SvJae|
|Molecular Note||Exons 5-8, including sequences encoding the putative pore region and part of the C-terminus of the protein, have been replaced by a neomycin resistance cassette.|
|Mutations Made By|| |
David Clapham, Janelia Research Campus, Howard Hughes Medical Institute
When maintained as a live colony, homozygous females are crossed with heterozygous males. Homozygous males are infertile.
When using the CatSper4- mouse strain in a publication, please cite the originating article(s) and include JAX stock #006821 in your Materials and Methods section.