Homozygous males of this Catsper4 knock-out mutation are infertile.
David Clapham, Janelia Research Campus, Howard Hughes Medical Institute
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Catsper4 | cation channel, sperm associated 4 |
Mice homozygous for this targeted mutation are viable and do not display any gross physical or behavioral abnormalities. Male homozygotes are infertile due to a defect in sperm motility. Their sperm fails to develop the hyperactive motility pattern required for fertilization. No morphological differences between wildtype and mutant spermatozoa have been observed. No protein product from the targeted gene is detected in testis tissue. Female homozygotes reproduce normally. This strain may be useful in studies of male infertility.
A targeting vector containing a neomycin resistance gene was used to replace exons 5-8. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with 129S1/Sv. The strain was backcrossed three times to 129S1/Sv by the donating laboratory.
Allele Name | targeted mutation 1, David E Clapham |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Catsper4, cation channel, sperm associated 4 |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 4 |
Molecular Note | Exons 5-8, including sequences encoding the putative pore region and part of the C-terminus of the protein, have been replaced by a neomycin resistance cassette. |
Mutations Made By | David Clapham, Janelia Research Campus, Howard Hughes Medical Institute |
When maintained as a live colony, homozygous females are crossed with heterozygous males. Homozygous males are infertile.
When using the CatSper4- mouse strain in a publication, please cite the originating article(s) and include JAX stock #006821 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wild-type for Catsper4<tm1Clph> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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