129S-Catsper4^tm1Clph/J

Stock number: 006821
Product name: CatSper4-
Research areas: Reproductive Biology Research

Description

Homozygous males of this Catsper4 knock-out mutation are infertile.

Detailed description

Mice homozygous for this targeted mutation are viable and do not display any gross physical or behavioral abnormalities. Male homozygotes are infertile due to a defect in sperm motility. Their sperm fails to develop the hyperactive motility pattern required for fertilization. No morphological differences between wildtype and mutant spermatozoa have been observed. No protein product from the targeted gene is detected in testis tissue. Female homozygotes reproduce normally. This strain may be useful in studies of male infertility.

Development

A targeting vector containing a neomycin resistance gene was used to replace exons 5-8. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with 129S1/Sv. The strain was backcrossed three times to 129S1/Sv by the donating laboratory.

Allele

Symbol: Catsper4^tm1Clph
Name: targeted mutation 1, David E Clapham
MGI accession ID: MGI:3694354
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Catsper4
Marker name: cation channel, sperm associated 4
Chromosome: 4
Strain of origin: 129S4/SvJae
Molecular note: Exons 5-8, including sequences encoding the putative pore region and part of the C-terminus of the protein, have been replaced by a neomycin resistance cassette.