B6;129P2-Del(14)3Mom/MomJ

Stock number: 006790
Product name: ΔH
Strain synonyms: DH

Description

Mice carrying this deletion exhibit loss or diminished expression of a subset of olfactory receptors that are on the same chromosome as the deletion, with less impact on olfactory receptor genes with increased chromosomal distance from the deletion.

Detailed description

The H element was identified by the high homoology of this non-coding DNA between mouse and human. In hemizygotes, the Del(14)3Mom deletion of the H element abolishes expression of Olfr1507 (MOR28) and Olfr1508 (MOR10) genes in cis from the 129P2/OlaHsd chromosome, while expression of Olfr1507 and Olfr1508 genes from the wild-type C57BL/6J chromosome persists. In homozygotes, Olfr1507, Olfr1508, and Olfr1509 (MOR83) genes are not expressed, and there is a graded, distance-related decrease in the numbers of cells expressing more distal olfactory receptor genes, Olfr1510 (MOR29A), Olfr1511 (MOR29B), Olfr1512 (MOR30A), and Olfr1513 (MOR30B). The expression of other olfactory receptor genes on Chromosome 14 such as Olfr1514 (MOR205-1), Olfr749 ((S1), and Olfr221 (MOR205-1) is not affected in an obvious way by the Del(14)3Mom deletion. Expression of olfactory receptor genes located on other chromosomes, including Olfr6 (M50), Olfr17 (P2), Olfr16 (MOR23), Olfr151 (M71), and Olfr15 (MOR256-17), is not impacted. Thus, deletion of the H element has a local and cis effect on the expression of olfactory receptor genes.

Development

A 2,119 nucleotide ScaI-flanked sequence of the H element was replaced with the ACNF neomycin resistance cassette via homologous recombination in 129P2/OlaHsd derived E14 ES cells and this neomycin resistance cassette self-excised in the male germline. Male chimeras were bred to C57BL/6J females. This line has been maintained on this mixed B6;129P2 background and was intercrossed to homozygosity. In 2007 sperm from homozygous males was cryopreserved.

The plasmid that was used to generate this mutation is banked and described at Addgene.

Allele

Symbol: Del(14)3Mom
Name: deletion, Chr 14, Peter Mombaerts 3
MGI accession ID: MGI:3714752
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Del(14)3Mom
Marker name: deletion, Chr 14, Peter Mombaerts 3
Chromosome: 14
Strain of origin: 129P2/OlaHsd
Molecular note: The H element, upstream of Olfr1507, was deleted by replacing the 2,119 nucleotide ScaI flanked segment with the ACNF neomycin resistance cassette via homologous recombination and the neomycin resistance cassette was self-excised during germline transmission.

Allele

MGI accession ID: MGI:5000535
Marker symbol: Rr12
Marker name: regulatory region 12
Chromosome: 14