These transgenic mice, carrying Cd80 in the construct do not develop diabetes, in contrast to similiar NOD mice carrying a transgene with Cd274 (Stock No. 012310).
Hans-Michael Dosch, University of Toronto
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Inserted expressed sequence) |
Gfap-Cd80 (GFAP-B7-1) transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. The donating investigator reports that transgenic mice are protected from diabetes development.
This transgenic strain carries a T cell co-stimulatory molecule, Cd80 (B7-1), cDNA fused to the glial fibrillary acidic protein (Gfap) promoter. The transgenic construct was injected into NOD/ShiLt fertilized embryos, and progeny of founder 9 were mated to NOD/ShiLt. In 2006, the T1DR received hemizygous mice that were mated to NOD/ShiLtJ (Stock No. 001976) prior to sibling mating.
Expressed Gene | CD80, CD80 molecule, human |
---|---|
Site of Expression |
Allele Name | transgene insertion 9, H Michael Dosch |
---|---|
Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | GFAP-B71; GFAP-B7-1Tg line 9; Tg(Gfap-CD80)9Mdos |
Gene Symbol and Name | Tg(GFAP-CD80)9Mdos, transgene insertion 9, H Michael Dosch |
Gene Synonym(s) | |
Promoter | GFAP, glial fibrillary acidic protein, human |
Expressed Gene | CD80, CD80 molecule, human |
Strain of Origin | NOD/ShiLt |
Chromosome | UN |
Molecular Note | This transgene contains cDNA encoding CD80 under control of the glial fibrillary acidic protein promoter. |
When using the NOD.GFAP-B7.1 mouse strain in a publication, please cite the originating article(s) and include JAX stock #006778 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(GFAP-Cd80)9Mdos |
Frozen Mouse Embryo | NOD/ShiLt-Tg(GFAP-Cd80)9Mdos/MdosJ | $2595.00 |
Frozen Mouse Embryo | NOD/ShiLt-Tg(GFAP-Cd80)9Mdos/MdosJ | $2595.00 |
Frozen Mouse Embryo | NOD/ShiLt-Tg(GFAP-Cd80)9Mdos/MdosJ | $3373.50 |
Frozen Mouse Embryo | NOD/ShiLt-Tg(GFAP-Cd80)9Mdos/MdosJ | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.