These transgenic mice with the rat insulin 2 promoter express Cd274 in pancreatic cells and develop accelerated diabetes.
Hans-Michael Dosch, University of Toronto
Genetic Background | Generation |
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001976 NOD/ShiLtJ |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
Ins2-Cd274 (Rip-B7H1) transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. The donating investigator indicates transgenic mice over express Cd274 in pancreatic beta cells and reports accelerated diabetes in Ins2-Cd274 transgenic mice compared to wildtype cohorts.
This strain is useful to study the role of Cd274 in autoimmunity and peripheral tolerance.
A member of the CD80/86 (B7) gene family, CD274, commonly called B7H1 or PD-L1, is normally expressed in antigen presenting cells and non-lymphoid tissues such as heart and lung. This transgenic strain carries a CD274 (B7H1) cDNA fused to thrat insulin promoter (Ins2) injected into NOD/ShiLt fertilized embryos. Progeny of founder 2 were mated to NOD/ShiLt. In 2006, the T1DR received hemizygous mice that were mated to NOD/ShiLtJ (Stock No. 001976) prior to sibling mating.
Expressed Gene | CD274, CD274 molecule, human |
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Site of Expression |
Allele Name | transgene insertion 2, H Michael Dosch |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | RIP-B7H1; RIP-PDL1L |
Gene Symbol and Name | Tg(Ins2-Cd274)2Mdos, transgene insertion 2, H Michael Dosch |
Gene Synonym(s) | |
Promoter | Ins2, insulin 2, rat |
Expressed Gene | CD274, CD274 molecule, human |
Strain of Origin | NOD/ShiLt |
Chromosome | UN |
Molecular Note | This transgene contains cDNA encoding human CD274 (B7H1) fused to the rat insulin 2 promoter. Transgenic mice express CD274 in pancreatic beta cells. |
Mutations Made By | Hans-Michael Dosch, University of Toronto |
When using the NOD.RIP-PDL1 mouse strain in a publication, please cite the originating article(s) and include JAX stock #006777 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(Ins2-Cd274)2Mdos |
Frozen Mouse Embryo | NOD/ShiLt-Tg(Ins2-Cd274)2Mdos/MdosJ | $2595.00 |
Frozen Mouse Embryo | NOD/ShiLt-Tg(Ins2-Cd274)2Mdos/MdosJ | $2595.00 |
Frozen Mouse Embryo | NOD/ShiLt-Tg(Ins2-Cd274)2Mdos/MdosJ | $3373.50 |
Frozen Mouse Embryo | NOD/ShiLt-Tg(Ins2-Cd274)2Mdos/MdosJ | $3373.50 |
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