NOD/ShiLt-Tg(Ins2-Cd274)2Mdos/MdosJ

Stock number: 006777
Product name: NOD.RIP-PDL1
Strain synonyms: NOD.RIP-B7H1, Rip-B7H1
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research

Description

These transgenic mice with the rat insulin 2 promoter express Cd274 in pancreatic cells and develop accelerated diabetes.

Detailed description

Ins2-Cd274 (Rip-B7H1) transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. The donating investigator indicates transgenic mice over express Cd274 in pancreatic beta cells and reports accelerated diabetes in Ins2-Cd274 transgenic mice compared to wildtype cohorts.

This strain is useful to study the role of Cd274 in autoimmunity and peripheral tolerance.

Development

A member of the CD80/86 (B7) gene family, CD274, commonly called B7H1 or PD-L1, is normally expressed in antigen presenting cells and non-lymphoid tissues such as heart and lung. This transgenic strain carries a CD274 (B7H1) cDNA fused to thrat insulin promoter (Ins2) injected into NOD/ShiLt fertilized embryos. Progeny of founder 2 were mated to NOD/ShiLt. In 2006, the T1DR received hemizygous mice that were mated to NOD/ShiLtJ (Stock No. 001976) prior to sibling mating.

Allele

Symbol: Tg(Ins2-Cd274)2Mdos
Name: transgene insertion 2, H Michael Dosch
MGI accession ID: MGI:3701452
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(Ins2-Cd274)2Mdos
Marker name: transgene insertion 2, H Michael Dosch
Chromosome: UN
Strain of origin: NOD/ShiLt
Expressed genes: CD274,CD274 molecule,human
Molecular note: This transgene contains cDNA encoding human CD274 (B7H1) fused to the rat insulin 2 promoter. Transgenic mice express CD274 in pancreatic beta cells.