B6;129P2-Or8a1^tm33Mom/MomJ

Stock number: 006746
Product name: M71-IRES-tauYFP
Strain synonyms: M71-IRES-tauYFP

Description

These IRES-tauYFP reporter mice are useful in studies to identify olfactory sensory neuronal development and topography in glomeruli in the olfactory bulb.

Detailed description

Olfactory sensory neurons that express the olfactory receptor Or8a1 (formerly Olfr151) also co-express the tauYFP fusion protein by virtue of IRES-mediated co-translation. These neurons can be revealed by the intrinsic fluorescence of YFP, or by immunofluorescence with anti-YFP antibodies.

Development

The Or8a1^tm33Mom allele (formerly Olfr151^tm33Mom, also known as M71-IRES-tauYFP) was generated in 129P2/OlaHsd derived E14 ES cells and resulting mice were bred to C57BL/6J before being intercrossed to homozygosity. This mutation was generated using the ACNf neomycin resistance cassette, which self-excises during transmission in the male chimera. Sperm was cryopreserved from homozygous males on this mixed B6;129P2 background.

Allele

Symbol: Or8a1^tm33Mom
Name: targeted mutation 33, Peter Mombaerts
MGI accession ID: MGI:3712831
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Or8a1
Marker name: olfactory receptor family 8 subfamily A member 1
Chromosome: 9
Strain of origin: 129P2/OlaHsd
Expressed genes: YFP,Yellow Fluorescent Protein,jellyfish; Or8a1,olfactory receptor family 8 subfamily A member 1,mouse, laboratory
Site of expression: Bicistronic expression of the endogenous gene, Olfr151, and tauYFP Is observed in olfactory sensory neurons.
Molecular note: An IRES-tauYFP and self-excising neomycin selection cassette were inserted downstream of the endogenous coding sequence, resulting in bicistronic expression of the endogenous gene and tauYFP.