A STOP-taulacZ cassette was introduced into the Gt(ROSA)26Sor locus by gene targeting. Upon expression of a site-specific Cre recombinase, the STOP segment is excised permanently, and expression of the fusion protein taulacZ is driven by the ROSA26 promoter. This fusion protein binds to microtubules and allows for the decoration of axons. It can be revealed by histochemistry for beta-galactosidase enzymatic activity, or immunohistochemistry with anti-beta-galactosidase antibodies.

This targeted insertion of a loxP-flanked STOP taulacZ cassette into the <i>Gt(ROSA)26Sor</i> locus provides a reporter allele for Cre recombinase expression in which taulacZ is expressed along the axons of neurons in which Cre recombinase has excised the loxP-flanked STOP.

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