This targeted insertion of a loxP-flanked STOP taulacZ cassette into the Gt(ROSA)26Sor locus provides a reporter allele for Cre recombinase expression in which taulacZ is expressed along the axons of neurons in which Cre recombinase has excised the loxP-flanked STOP.
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), Reporter) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
A STOP-taulacZ cassette was introduced into the Gt(ROSA)26Sor locus by gene targeting. Upon expression of a site-specific Cre recombinase, the STOP segment is excised permanently, and expression of the fusion protein taulacZ is driven by the ROSA26 promoter. This fusion protein binds to microtubules and allows for the decoration of axons. It can be revealed by histochemistry for beta-galactosidase enzymatic activity, or immunohistochemistry with anti-beta-galactosidase antibodies.
This allele was generated in E14 ES cells and the founder was bred once to C57BL/6J after which the line was maintained by sibling intercrossing and bred to homozygosity for this allele. In 2007 sperm was cryopreserved from homozygous males on this mixed B6;129P2 background.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | When bred with mice containing Cre recombinase, lacZ will be expressed in Cre-expressing cells of the offspring. |
Allele Name | targeted mutation 1, Peter Mombaerts |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter) |
Allele Synonym(s) | ROSA26-STOP-taulacZ |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | When bred with mice containing Cre recombinase, lacZ will be expressed in Cre-expressing cells of the offspring. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 6 |
Molecular Note | A splice acceptor containing Asc1 (CTAGTTCCCTTTTTTTTCACAG-GTTGGCGCGCCACGGTGATC) was inserted in the Nhe site of the intron of Gt(ROSA)26Sor, and then, into the Asc1 site, a sequence containing Asc1-loxP-CAT-pA-loxP-tauLacZ-betaGlobinpA-FRT-Neo-FRT-Asc1 was inserted to generate this allele with a loxP-flanked STOP sequence just upstream of tauLacZ. |
When using the ROSA-STOP-taulacZ mouse strain in a publication, please cite the originating article(s) and include JAX stock #006744 in your Materials and Methods section.
Facility Barrier Level Descriptions
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Heterozygous for Gt(ROSA)26Sor<tm1.1Mom> |
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