B6;129P2-Gt(ROSA)26Sor^tm1Mom/MomJ

Stock number: 006744
Product name: ROSA-STOP-taulacZ
Research areas: Developmental Biology Research, Neurobiology Research, Research Tools, Sensorineural Research

Description

This targeted insertion of a loxP-flanked STOP taulacZ cassette into the Gt(ROSA)26Sor locus provides a reporter allele for Cre recombinase expression in which taulacZ is expressed along the axons of neurons in which Cre recombinase has excised the loxP-flanked STOP.

Detailed description

A STOP-taulacZ cassette was introduced into the Gt(ROSA)26Sor locus by gene targeting. Upon expression of a site-specific Cre recombinase, the STOP segment is excised permanently, and expression of the fusion protein taulacZ is driven by the ROSA26 promoter. This fusion protein binds to microtubules and allows for the decoration of axons. It can be revealed by histochemistry for beta-galactosidase enzymatic activity, or immunohistochemistry with anti-beta-galactosidase antibodies.

Development

This allele was generated in E14 ES cells and the founder was bred once to C57BL/6J after which the line was maintained by sibling intercrossing and bred to homozygosity for this allele. In 2007 sperm was cryopreserved from homozygous males on this mixed B6;129P2 background.

Allele

Symbol: Gt(ROSA)26Sor^tm1Mom
Name: targeted mutation 1, Peter Mombaerts
MGI accession ID: MGI:3712829
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); Reporter
Marker symbol: Gt(ROSA)26Sor
Marker name: gene trap ROSA 26, Philippe Soriano
Chromosome: 6
Strain of origin: 129P2/OlaHsd
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: When bred with mice containing Cre recombinase, lacZ will be expressed in Cre-expressing cells of the offspring.
Molecular note: A splice acceptor containing Asc1 (CTAGTTCCCTTTTTTTTCACAG-GTTGGCGCGCCACGGTGATC) was inserted in the Nhe site of the intron of Gt(ROSA)26Sor, and then, into the Asc1 site, a sequence containing Asc1-loxP-CAT-pA-loxP-tauLacZ-betaGlobinpA-FRT-Neo-FRT-Asc1 was inserted to generate this allele with a loxP-flanked STOP sequence just upstream of tauLacZ.