GFP is expressed in vomeronasal sensory neurons in mice of this Vmn2r26tm2Mom knockout.
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Vmn2r26 | vomeronasal 2, receptor 26 |
Although the Vmn2r26tm2Mom allele does not express VMN2R26 protein, the vomeronasal sensory neurons actively transcribing this locus express green fluorescent protein. These neurons fail to respond, even at high concentrations, to MHC peptides or urine that stimulate VMN2R26-expressing vomeronasal sensory neurons. However, these non-responsive neurons will flux calcium normally in response to high potassium or a diacylglycerol analog, indicating normal signaling capacity.
This targeted mutation was generated in 129P2/OlaHsd derived E14 ES cells and the ACNF neomycin cassette was self-excised by cre recombination in the germline of male chimeras. This line was subsequently bred to C57BL/6J and then intercrossed. In 2007 sperm were cryopreserved from homozygous males.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | GFP is expressed in vomeronasal sensory neurons. |
Allele Name | targeted mutation 2, Peter Mombaerts |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | deltaV2r1b-GFP; deltaV2r1b-IRES-tauGFP |
Gene Symbol and Name | Vmn2r26, vomeronasal 2, receptor 26 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | GFP is expressed in vomeronasal sensory neurons. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 6 |
Molecular Note | Exon 6, which encodes seven putative transmembrane helices, was replaced by an IRES-tauGFP cassette. This allele fails to express the endogenous protein but expresses GFP in vomeronasal sensory neurons actively transcribing this locus. |
Mutations Made By | Peter Mombaerts, Max Planck Research Unit for Neurogenetics |
When using the V2r1b-GFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #006728 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Vmn2r26<tm2Mom> |
Frozen Mouse Embryo | B6;129P2-Vmn2r26<tm2Mom>/MomJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Vmn2r26<tm2Mom>/MomJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Vmn2r26<tm2Mom>/MomJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129P2-Vmn2r26<tm2Mom>/MomJ Frozen Embryo | $3373.50 |
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