B6;129P2-Vmn2r26^tm2Mom/MomJ

Stock number: 006728
Product name: V2r1b-GFP
Research areas: Neurobiology Research, Research Tools, Sensorineural Research

Description

GFP is expressed in vomeronasal sensory neurons in mice of this Vmn2r26^tm2Mom knockout.

Detailed description

Although the Vmn2r26^tm2Mom allele does not express VMN2R26 protein, the vomeronasal sensory neurons actively transcribing this locus express green fluorescent protein. These neurons fail to respond, even at high concentrations, to MHC peptides or urine that stimulate VMN2R26-expressing vomeronasal sensory neurons. However, these non-responsive neurons will flux calcium normally in response to high potassium or a diacylglycerol analog, indicating normal signaling capacity.

Development

This targeted mutation was generated in 129P2/OlaHsd derived E14 ES cells and the ACNF neomycin cassette was self-excised by cre recombination in the germline of male chimeras. This line was subsequently bred to C57BL/6J and then intercrossed. In 2007 sperm were cryopreserved from homozygous males.

Allele

Symbol: Vmn2r26^tm2Mom
Name: targeted mutation 2, Peter Mombaerts
MGI accession ID: MGI:3722386
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Vmn2r26
Marker name: vomeronasal 2, receptor 26
Chromosome: 6
Strain of origin: 129P2/OlaHsd
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: GFP is expressed in vomeronasal sensory neurons.
Molecular note: Exon 6, which encodes seven putative transmembrane helices, was replaced by an IRES-tauGFP cassette. This allele fails to express the endogenous protein but expresses GFP in vomeronasal sensory neurons actively transcribing this locus.