B6;129P2-Vmn2r81^tm2Mom/MomJ

Stock number: 006726
Product name: V2rf2-GFP
Research areas: Research Tools

Description

The reporter targeted mutation in these mice contains beta-galactosidase that can be visualized in vomeronasal sensory neurons by histochemistry or immunofluorescence with anti-beta-galactosidase antibodies.

Detailed description

Olfactory sensory neurons that express the vomeronasal receptor Vmn2r81 also co-express the taulacZ fusion protein by virtue of IRES-mediated co-translation. These neurons can be revealed by histochemistry of beta-galactosidase enzymatic activity, or by immunofluorescence with anti-beta-galactosidase antibodies.

Development

This mutation was generated in 129P2/OlaHsd derived E14 ES cells and the ACNf neomycin cassette self-excised by cre recombinantion in the germline of the male chimeras. Founders were bred with C57BL/6J. Sperm was cryopreserved from homozygous males on this mixed B6;129P2 background.

Allele

Symbol: Vmn2r81^tm2Mom
Name: targeted mutation 2, Peter Mombaerts
MGI accession ID: MGI:3722385
Generation method: Targeted
Attributes: Reporter
Marker symbol: Vmn2r81
Marker name: vomeronasal 2, receptor 81
Chromosome: 10
Strain of origin: 129P2/OlaHsd
Expressed genes: GFP,Green Fluorescent Protein,; Vmn2r81,vomeronasal 2, receptor 81,mouse, laboratory
Site of expression: TaulacZ fusion protein is expressed in olfactory sensory neurons.
Molecular note: An IRES-tauGFP-ACNF cassette was inserted after the stop codon resulting in bicistronic expression of green fluorescent protein with the endogenous gene.