B6;129P2-Olfr545^tm1Mom/MomJ

Stock number: 006712
Product name: S50-IRES-tauGFP
Strain synonyms: S50-IRES-tauGFP, clone S50G-37
Research areas: Developmental Biology Research, Neurobiology Research, Research Tools, Sensorineural Research

Description

The class I olfactory receptor Olfr545 gene in the targeted reporter allele of these mice is labeled with green fluorescent protein and expression is typically projected to a single dorsal-medial and a single anterior-lateral glomerulus per olfactory bulb.

Detailed description

The Olfr545^tm1Mom allele has a fully intact coding sequence with a tauGFP tag permitting bicistronic expression of the endogenous gene and the green fluorescent protein tag. The olfactory sensory neurons that express this tagged allele typically project to a single dorsal-medial and a single anterior-lateral glomerulus per olfactory bulb, and, when co-expressed with Tg(P-taulacZ)8Mom expression is found not to overlap.

Development

An 8.2 kb BamH1/BglII genomic fragment containing Olfr545 was isolated from a 129/SvJ BAC clone, an IRES-tauGFP was inserted 3 nucleotides downstream of the stop codon, along with the self-excising ACNf neomycin resistance cassette. The construct was used for homologous recombination in 129P2/OlaHsd derived E14 ES cells and a founder from clone S50G-37 was bred to C57BL/6J females.

Allele

Symbol: Olfr545^tm1Mom
Name: targeted mutation 1, Peter Mombaerts
MGI accession ID: MGI:3722364
Generation method: Targeted
Attributes: Reporter
Synonyms: S50-IRES-tauGFP
Marker symbol: Olfr545
Marker name: olfactory receptor 545
Chromosome: 7
Strain of origin: 129P2/OlaHsd
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: olfactory sensory neurons
Molecular note: An IRES-tauGFP and self-excising neomycin resistance casette were inserted downstream of the Olfr545 coding sequence resulting in bicistronic expression of Olfr545 and green fluorescent protein. Clone S50G-37 was used to generate this allele.