The class I olfactory receptor Olfr545 gene in the targeted reporter allele of these mice is labeled with green fluorescent protein and expression is typically projected to a single dorsal-medial and a single anterior-lateral glomerulus per olfactory bulb.
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Reporter) | Olfr545 | olfactory receptor 545 |
The Olfr545tm1Mom allele has a fully intact coding sequence with a tauGFP tag permitting bicistronic expression of the endogenous gene and the green fluorescent protein tag. The olfactory sensory neurons that express this tagged allele typically project to a single dorsal-medial and a single anterior-lateral glomerulus per olfactory bulb, and, when co-expressed with Tg(P-taulacZ)8Mom expression is found not to overlap.
An 8.2 kb BamH1/BglII genomic fragment containing Olfr545 was isolated from a 129/SvJ BAC clone, an IRES-tauGFP was inserted 3 nucleotides downstream of the stop codon, along with the self-excising ACNf neomycin resistance cassette. The construct was used for homologous recombination in 129P2/OlaHsd derived E14 ES cells and a founder from clone S50G-37 was bred to C57BL/6J females.
Expressed Gene | GFP, Green Fluorescent Protein, |
---|---|
Site of Expression | olfactory sensory neurons |
Allele Name | targeted mutation 1, Peter Mombaerts |
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Allele Type | Targeted (Reporter) |
Allele Synonym(s) | S50-IRES-tauGFP |
Gene Symbol and Name | Olfr545, olfactory receptor 545 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | olfactory sensory neurons |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 7 |
Molecular Note | An IRES-tauGFP and self-excising neomycin resistance casette were inserted downstream of the Olfr545 coding sequence resulting in bicistronic expression of Olfr545 and green fluorescent protein. Clone S50G-37 was used to generate this allele. |
When using the S50-IRES-tauGFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #006712 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Olfr545<tm1Mom> |
Frozen Mouse Embryo | B6;129P2-Olfr545<tm1Mom>/MomJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Olfr545<tm1Mom>/MomJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Olfr545<tm1Mom>/MomJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129P2-Olfr545<tm1Mom>/MomJ Frozen Embryo | $3373.50 |
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