B6;129P2-Or11h4^tm1Mom/MomJ

Stock number: 006711
Research areas: Neurobiology Research, Research Tools, Sensorineural Research

Description

This targeted reporter is valuable in the research of olfactory sensory neuron development and function.

Detailed description

This targeted reporter has bicistronic expression of the endogenous Or11h4 (formerly Olfr749) and tauGFP. GFP expression is found sparsely distributed in the dorsal main olfactory epithelium at 9 days of age and embryonic immunohistochemistry shows a steady increase in the number of olfactory sensory neurons (OSNs) expressing GFP from E15.5 to birth. There are approximately 95 GFP positive OSNs at embryonic day 15.5, approximately 500 at birth, and approximately 8,450 at 24 days of age. At embryonic day 15.5 the axons of these OR11H4 OSNs extend to the protoglomerular layer in the medial olfactory bulb, by E16.5 they form a web-like appearance, the formation of discrete medial glomeruli is seen after birth, and the medial glomerular diameter increases from perinatal day 1 through 24. This allele has been used to characterize the onset of prenatal odorant responsiveness by olfactory sensory neurons bearing OLFR749. Patch-clamp recordings showed that by embryonic day 16.5 these olfactory sensory neurons respond to 2-phenylethyl alcohol.

Development

This targeted insertion was generated in 129P2-derived E14 ES cells and after an outcross to C57BL/6J this strain was maintained by sibling breeding on this mixed background. Sperm was cryopreserved from homozygous males.

Allele

Symbol: Or11h4^tm1Mom
Name: targeted mutation 1, Peter Mombaerts
MGI accession ID: MGI:3722363
Generation method: Targeted
Attributes: Reporter
Marker symbol: Or11h4
Marker name: olfactory receptor family 11 subfamily H member 4
Chromosome: 14
Strain of origin: 129P2/OlaHsd
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: GFP expression is found sparsely distributed in the dorsal main olfactory epithelium at 9 days of age and embryonic immunohistochemistry shows a steady increase in the number of olfactory sensory neurons (OSNs) expressing GFP from E15.5 to birth. There are approximately 95 GFP positive OSNs at embryonic day 15.5, approximately 500 at birth, and approximately 8,450 at 24 days of age. At embryonic day 15.5 the axons of these OLFR749 OSNs extend to the protoglomerular layer in the medial olfactory bulb, by E16.5 they form a web-like appearance, the formation of discrete medial glomeruli is seen after birth, and the medial glomerular diameter increases from perinatal day 1 through 24.
Molecular note: An IRES-tauGFP-ACNF cassette was inserted 3 nucleotides downstream of the stop codon to yield bi-cistronic expression of tauGFP with the endogenous olfactory receptor protein. The neomycin selection sequence self-excised during male germline transmission.