These mice carry a targeted mutation of the semaphorin 3F gene. Axonal wiring or some vomeronasal sensory neurons is perturbed by the deficiency. This strain may be useful in studies of olfactory neurobiology.
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Sema3f | sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F |
These mice carry a targeted mutation of the semaphorin 3F gene. Axonal wiring or some vomeronasal sensory neurons is perturbed by the deficiency. Homozygotes are smaller than wildtype mice during the first few weeks after birth. Fewer homozygotes than predicted by Mendelian ratios are produced from heterozygous crosses. This strain may be useful in studies of olfactory neurobiology.
Exons 2-15 of the gene were replaced with a self-excising pgk-neomycin expression cassette flanked by loxP sites (ACNF). A single loxP site remains. This mutation was created in 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. This strain was maintained on a mixed C57BL/6J and 129P2/OlaHsd background by the donating investigator.
Allele Name | targeted mutation 1, Peter Mombaerts |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | deltaF |
Gene Symbol and Name | Sema3f, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 9 |
Molecular Note | 85% of the open reading frame, exons 2-15, were replaced with the ACNF cassette, which self-excised upon germline transmission leaving a null allele. |
Mutations Made By | Peter Mombaerts, Max Planck Research Unit for Neurogenetics |
When using the B6;129P2-Sema3ftm1Mom/MomJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #006710 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Sema3f<tm1Mom> |
Frozen Mouse Embryo | B6;129P2-Sema3f<tm1Mom>/MomJ | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Sema3f<tm1Mom>/MomJ | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Sema3f<tm1Mom>/MomJ | $3373.50 |
Frozen Mouse Embryo | B6;129P2-Sema3f<tm1Mom>/MomJ | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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