B6;129P2-Sema3f^tm1Mom/MomJ

Stock number: 006710
Research areas: Sensorineural Research

Description

These mice carry a targeted mutation of the semaphorin 3F gene. Axonal wiring or some vomeronasal sensory neurons is perturbed by the deficiency. This strain may be useful in studies of olfactory neurobiology.

Detailed description

These mice carry a targeted mutation of the semaphorin 3F gene. Axonal wiring or some vomeronasal sensory neurons is perturbed by the deficiency. Homozygotes are smaller than wildtype mice during the first few weeks after birth. Fewer homozygotes than predicted by Mendelian ratios are produced from heterozygous crosses. This strain may be useful in studies of olfactory neurobiology.

Development

Exons 2-15 of the gene were replaced with a self-excising pgk-neomycin expression cassette flanked by loxP sites (ACNF). A single loxP site remains. This mutation was created in 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. This strain was maintained on a mixed C57BL/6J and 129P2/OlaHsd background by the donating investigator.

Allele

Symbol: Sema3f^tm1Mom
Name: targeted mutation 1, Peter Mombaerts
MGI accession ID: MGI:3758889
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: deltaF
Marker symbol: Sema3f
Marker name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F
Marker synonyms: Sema IV; SEMA4; SemaIV; SEMA-IV; SEMAK
Chromosome: 9
Strain of origin: 129P2/OlaHsd
Molecular note: 85% of the open reading frame, exons 2-15, were replaced with the ACNF cassette, which self-excised upon germline transmission leaving a null allele.