Expression of S100a5 is upregulated in olfactory sensory neurons in response to stimulation. This reporter null allele allows activity-dependent expression of GFP from the S100a5 locus such that when a specific olfactory sensory neuron is stimulated through its olfactory receptor that neuron can be detected by its GFP expression. This permits in vivo tracking of which olfactory receptor responds to which odorant. As expected from the lack of S100a5 transcript in vomeronasal sensory neurons, this allele does not drive GFP expression in vomeronasal sensory neurons. In their proof-of-concept paper McClintock et al. used this system to track eugenol response to olfactory sensory neurons expressing Olfr73, Olfr178, Olfr432, Olfr610, Olfr958, Olfr960, Olfr961, Olfr913, and Olfr1234 and muscone response to olfactory sensory neurons expressing Olfr74, Olfr235, Olfr816, and Olfr1440. When crossed with Cnga2tm1Mom mice, which have odorant-evoked electrical activity ablated, the progeny null for CNGA2 have nearly undetectable GFP fluorescence from the S100a5 GFP reporter.

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This reporter null allele is valuable for mapping responses of specific olfactory receptors to specific odorants.

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STOCK S100a5<tm1.1Mom>/MomJ Frozen Embryo