Expression of the semaphorin 3B gene is blocked by the removal of the entire open reading frame. Homozygotes are viable, appear normal and are born at Mendelian frequencies. This strain may be useful in studies of axonal patterning in the olfactory nervous system.
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Sema3b | sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B |
Expression of the semaphorin 3B gene is blocked by the removal of the entire open reading frame. Homozygotes are viable, appear normal and are born at Mendelian frequencies. This strain may be useful in studies of axonal patterning in the olfactory nervous system.
A self-excising pgk-neomycin expression cassette flanked by loxP sites was used to replace exons 1-17 of the gene. A single loxP site remains. The mutation was created in 129P2/OlaHsd-derived E14.1 embryonic stem (ES) cells. The mutation was maintained on a mixed C57BL/6J and 129P2/OlaHsd genetic background by the donating laboratory.
Allele Name | targeted mutation 1, Peter Mombearts |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | deltaB |
Gene Symbol and Name | Sema3b, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 9 |
Molecular Note | All of the open reading frame, exons 1-17, were replaced with the ACNF cassette, which self-excised upon germline transmission leaving a null allele. |
Mutations Made By | Peter Mombaerts, Max Planck Research Unit for Neurogenetics |
When using the B6;129P2-Sema3btm1Mom/MomJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #006705 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Sema3b<tm1Mom> |
Frozen Mouse Embryo | B6;129P2-Sema3b<tm1Mom>/MomJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Sema3b<tm1Mom>/MomJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Sema3b<tm1Mom>/MomJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129P2-Sema3b<tm1Mom>/MomJ Frozen Embryo | $3373.50 |
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