B6;129P2-Sema3b^tm1Mom/MomJ

Stock number: 006705
Research areas: Sensorineural Research

Description

Expression of the semaphorin 3B gene is blocked by the removal of the entire open reading frame. Homozygotes are viable, appear normal and are born at Mendelian frequencies. This strain may be useful in studies of axonal patterning in the olfactory nervous system.

Detailed description

Expression of the semaphorin 3B gene is blocked by the removal of the entire open reading frame. Homozygotes are viable, appear normal and are born at Mendelian frequencies. This strain may be useful in studies of axonal patterning in the olfactory nervous system.

Development

A self-excising pgk-neomycin expression cassette flanked by loxP sites was used to replace exons 1-17 of the gene. A single loxP site remains. The mutation was created in 129P2/OlaHsd-derived E14.1 embryonic stem (ES) cells. The mutation was maintained on a mixed C57BL/6J and 129P2/OlaHsd genetic background by the donating laboratory.

Allele

Symbol: Sema3b^tm1Mom
Name: targeted mutation 1, Peter Mombearts
MGI accession ID: MGI:3758884
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Sema3b
Marker name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B
Chromosome: 9
Strain of origin: 129P2/OlaHsd
Molecular note: All of the open reading frame, exons 1-17, were replaced with the ACNF cassette, which self-excised upon germline transmission leaving a null allele.