Green fluorescent protein in these targeted neurotensin mutant mice marks cells of the main olfactory bulb and their axons.
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Reporter) | Nts | neurotensin |
Through bicistronic expression of enhanced green fluorescing protein and neurotensin, this strain permits the visualization of the mitral and tufted cells of the main olfactory bulb thereby permitting developmental analysis in the lateral olfactory tract.
The Ntstm1Mom mutation was generated in 129P2/OlaHsd derived E14 ES cells and the pgk-neomycin expression cassette flanked by loxP sites (LNL) was excised by breeding to mice carrying the Tg(EIIa-cre)Lmgd transgene, which was generated in FVB/N but was backcrossed onto C57BL/6J at least four times before this cross. The transgene was selectively bred out of the resulting line.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | Green fluorescence observed in mitral and tufted cells of the main olfactory bulb. |
Allele Name | targeted mutation 1, Peter Mombaerts |
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Allele Type | Targeted (Reporter) |
Allele Synonym(s) | Neurotensin-IRES-tauGFP; NT-GFP |
Gene Symbol and Name | Nts, neurotensin |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | Green fluorescence observed in mitral and tufted cells of the main olfactory bulb. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 10 |
Molecular Note | An IRES-tauGFP and a pgk-neomycin cassette flanked by loxP sites were inserted 3 bp after the stop codon. The neo cassette was later removed via Cre mediated recombination. Both Nts and tauGFP are expressed. |
Mutations Made By | Peter Mombaerts, Max Planck Research Unit for Neurogenetics |
This strain can be maintained by intercrossing homozygotes
When using the NT-GFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #006702 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Nts<tm1Mom> |
Frozen Mouse Embryo | STOCK Nts<tm1Mom>/MomJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Nts<tm1Mom>/MomJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Nts<tm1Mom>/MomJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Nts<tm1Mom>/MomJ Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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