STOCK Nts^tm1Mom/MomJ

Stock number: 006702
Product name: NT-GFP
Strain synonyms: Neurotensin-IRES-tauGFP
Research areas: Developmental Biology Research, Research Tools, Sensorineural Research

Description

Green fluorescent protein in these targeted neurotensin mutant mice marks cells of the main olfactory bulb and their axons.

Detailed description

Through bicistronic expression of enhanced green fluorescing protein and neurotensin, this strain permits the visualization of the mitral and tufted cells of the main olfactory bulb thereby permitting developmental analysis in the lateral olfactory tract.

Development

The Nts^tm1Mom mutation was generated in 129P2/OlaHsd derived E14 ES cells and the pgk-neomycin expression cassette flanked by loxP sites (LNL) was excised by breeding to mice carrying the Tg(EIIa-cre)Lmgd transgene, which was generated in FVB/N but was backcrossed onto C57BL/6J at least four times before this cross. The transgene was selectively bred out of the resulting line.

Allele

Symbol: Nts^tm1Mom
Name: targeted mutation 1, Peter Mombaerts
MGI accession ID: MGI:3695392
Generation method: Targeted
Attributes: Reporter
Marker symbol: Nts
Marker name: neurotensin
Chromosome: 10
Strain of origin: 129P2/OlaHsd
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: Green fluorescence observed in mitral and tufted cells of the main olfactory bulb.
Molecular note: An IRES-tauGFP and a pgk-neomycin cassette flanked by loxP sites were inserted 3 bp after the stop codon. The neo cassette was later removed via Cre mediated recombination. Both Nts and tauGFP are expressed.