STOCK Or8a1^tm20Mom/MomJ

Stock number: 006694
Product name: M71(ΔM1)-LacZ
Strain synonyms: M71(DM1)-IRES-taulacZ
Research areas: Research Tools, Sensorineural Research

Development

This targeted mutation was generated in 129P2/OlaHsd derived E14 ES cells and the resulting mice were crossed to Tg(EIIa-cre) bearing mice to excise the loxP flanked pgkneo (LNL) cassette. Although this transgene was generated in FVB/N mice, it was backcrossed onto C57BL/6J prior to this cross. The offspring were intercrossed and the transgene was selectively bred out of this line. Thus, this STOCK background is predominantly C57BL/6J and 129P2/OlaHsd. In 2007 sperm were cryopreserved from homozygous males.

Allele

Symbol: Or8a1^tm20Mom
Name: targeted mutation 20, Peter Mombaerts
MGI accession ID: MGI:3047227
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Or8a1
Marker name: olfactory receptor family 8 subfamily A member 1
Chromosome: 9
Strain of origin: 129P2/OlaHsd
Expressed genes: lacZ,beta-galactosidase,E. coli
Molecular note: The 3rd bp (G) from the initiation methionine was deleted. No upstream ATGs were created in-frame, leaving the next potential ATG in the coding region at position 115, which would result in a protein truncated by 38 amino acids. An IRES-taulacZ was incorporated downstream of the coding sequence.