STOCK Or6b9^tm1Mom/MomJ

Stock number: 006686
Product name: M50iGFPiLacZ
Strain synonyms: M50-IRES-GFP-IRES-taulacZ

Description

This bicistronic IRES-tauLacZ reporter of Or6b9 (formerly Olfr6) expression is useful in studies to identify olfactory sensory neuronal development and topography in glomeruli in the olfactory bulb.

Detailed description

An IRES-tauLacZ reporter sequence was inserted just downstream of the expressed sequence for this olfactory receptor. Olfactory sensory neurons that express Or6b9 (formerly Olfr6) co-express tauLacZ by virtue of IRES-mediated co-translation.

Development

This targeted mutation was generated in 129P2/OlaHsd derived E14 ES cells and the resulting mice from clone MF219 were crossed to Tg(EIIa-cre) bearing mice to excise the loxP flanked pgkneo (LNL) cassette. Although this transgene was generated in FVB/N mice, it was backcrossed onto C57BL/6J prior to this cross. The offspring were intercrossed and the transgene was selectively bred out of this line. Thus, this STOCK background is predominantly C57BL/6J and 129P2/OlaHsd. In 2007 sperm were cryopreserved from homozygous males.

The plasmid that was used to generate this mutation is banked and described at Addgene.

Allele

Symbol: Or6b9^tm1Mom
Name: targeted mutation 1, Peter Mombaerts
MGI accession ID: MGI:3693438
Generation method: Targeted
Attributes: Reporter
Marker symbol: Or6b9
Marker name: olfactory receptor family 6 subfamily B member 9
Chromosome: 7
Strain of origin: 129P2/OlaHsd
Expressed genes: GFP,Green Fluorescent Protein,; lacZ,beta-galactosidase,E. coli
Site of expression: GFP and lacZ are expressed in olfactory sensory neurons most ventrally in the olfactory bulb.
Molecular note: An IRES-GFP-IRES-taulacZ cassette was inserted downstream of the endogenous coding sequence and breeding to EIIa-cre mice excised a floxed neo at the 3' end of the inserted cassette.