These GFP reporter mice are useful in studies to identify olfactory sensory neuronal development and topography in glomeruli in the olfactory bulb.
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Reporter) | Olfr151 | olfactory receptor 151 |
Olfactory sensory neurons that express the olfactory receptor Olfr151 also co-express the tauGFP fusion protein by virtue of IRES-mediated co-translation. These neurons can be revealed by the intrinsic fluorescence of GFP, or by immunofluorescence with anti-GFP antibodies.
Homologous recombination to generate this allele was done in 129P2/OlaHsd derived E14 ES cells. The loxP-flanked neo cassette was then removed by transient transfection of cre recombinase prior to injection of these ES cells into blastocysts. The resulting mice were bred to C57BL/6 and intercrossed.
The plasmid that was used to generate this mutation is banked and described at Addgene.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | The tauGFP fusion protein is expressed in olfactory sensory neurons that express the olfactory receptor Olfr151. |
Allele Name | targeted mutation 26, Peter Mombaerts |
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Allele Type | Targeted (Reporter) |
Allele Synonym(s) | M71-G; M71-GFP; M71-IRES-tauGFP; Olfr7tm2Mom |
Gene Symbol and Name | Olfr151, olfactory receptor 151 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | The tauGFP fusion protein is expressed in olfactory sensory neurons that express the olfactory receptor Olfr151. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 9 |
Molecular Note | An IRES-tauGFP gene was inserted 3 nucleotides downstream of the endogenous stop codon. Crossing with EIIa-Cre mice excised a floxed neo at the 3' end of the inserted cassette. |
When using the M71-GFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #006676 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Olfr151<tm26Mom> |
Frozen Mouse Embryo | B6;129P2-Olfr151<tm26Mom>/MomJ | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Olfr151<tm26Mom>/MomJ | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Olfr151<tm26Mom>/MomJ | $3373.50 |
Frozen Mouse Embryo | B6;129P2-Olfr151<tm26Mom>/MomJ | $3373.50 |
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