B6;129P2-Or8a1^tm26Mom/MomJ

Stock number: 006676
Product name: M71-GFP
Strain synonyms: M71-IRES-tauGFP
Research areas: Research Tools, Sensorineural Research

Description

These GFP reporter mice are useful in studies to identify olfactory sensory neuronal development and topography in glomeruli in the olfactory bulb.

Detailed description

Olfactory sensory neurons that express the olfactory receptor Or8a1 (formerly Olfr151) also co-express the tauGFP fusion protein by virtue of IRES-mediated co-translation. These neurons can be revealed by the intrinsic fluorescence of GFP, or by immunofluorescence with anti-GFP antibodies. Heterozygotes and homozygotes are viable and fertile.

Development

An IRES-tauGFP cassette was inserted 3 nucleotides downstream of the endogenous Or8a1 (formerly Olfr151) stop codon via homologous recombination in 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. A loxP-flanked neomycin cassette at the 3’ end of the insertion was excised from the ES cells by transient transfection with cre recombinase. Resultant mice were bred with C57BL/6 mice and intercrossed by the donating laboratory.

The plasmid that was used to generate this mutation is banked and described at Addgene.

Allele

Symbol: Or8a1^tm26Mom
Name: targeted mutation 26, Peter Mombaerts
MGI accession ID: MGI:3051336
Generation method: Targeted
Attributes: Reporter
Marker symbol: Or8a1
Marker name: olfactory receptor family 8 subfamily A member 1
Chromosome: 9
Strain of origin: 129P2/OlaHsd
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: The tauGFP fusion protein is expressed in olfactory sensory neurons that express the olfactory receptor Olfr151.
Molecular note: An IRES-tauGFP gene was inserted 3 nucleotides downstream of the endogenous stop codon. Crossing with EIIa-Cre mice excised a floxed neo at the 3' end of the inserted cassette.