B6;CBA-Tg(Olfr16*,taulacZ)5Mom/MomJ

Stock number: 006671
Product name: MOR23-TgΔ-5-LacZ
Strain synonyms: MOR23-Tg(Delta)-IRES-tauLacZ (line TgDelta-5)

Development

A 9.4 kb Sac1-Nhe1 fragment containing Olfr16 was subcloned from a 129/Sv-derived P1 phage into pBluescript, the second intron was excised, and an IRES-taulacZ sequence was inserted just downstream of the coding sequence. This resulted in a 4.9 kb construct that was microinjected into (C57BL/6J x CBA)F2 zygotes and integration by random insertion occurred. Several lines were generated, and line 5, Tg(Olfr16*,taulacZ)5Mom, has a copy number of 15 to 20. This transgene has been maintained on a mixed B6;CBA background by sibling inbreeding.

Allele

Symbol: Tg(Olfr16*,taulacZ)5Mom
Name: transgene insertion 5, Peter Mombaerts
MGI accession ID: MGI:3692317
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Olfr16*,taulacZ)5Mom
Marker name: transgene insertion 5, Peter Mombaerts
Chromosome: UN
Strain of origin: Not Applicable
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: lacZ is expressed in olfactory sensory neurons on the turbinate epithelium.
Molecular note: This transgene resulted from pronuclear injection of a construct comprised of an IRES-taulacZ cassette downstream of a truncated Olfr16 sequence, with the three Olfr16 exons present but both intron 1 and intron 2 absent and 395 base pairs 5Õ of the translation start site deleted. The loss of these 395 base pairs stops expression.