These GFP reporter mice are useful in studies to identify olfactory sensory neuronal development and topography in glomeruli in the olfactory bulb.
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter) | Olfr17 | olfactory receptor 17 |
Olfactory sensory neurons that express the olfactory receptor Olfr17 also co-express the tauGFP fusion protein by virtue of IRES-mediated co-translation. These neurons can be revealed by the intrinsic fluorescence of GFP, or by immunofluorescence with anti-GFP antibodies.
Homologous recombination to generate this allele was done in 129P2/OlaHsd derived E14 ES cells. To excise the loxP-flanked neomycin resistance sequence, the resulting mouse was bred with a mouse carrying an EIIa-cre transgene (Lakso et al. 1996, Proc Natl Acad Sci 93:5860), a transgene generated in FVB/N but having been backcrossed at least four generations to C57BL/6 before this cross and having unknown pedigree prior to that. The resulting mice, lacking the neomycin resistance cassette, were intercrossed and homozygotes were selected that were devoid of the EIIa-cre transgene.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | The tauGFP fusion protein is expressed in olfactory sensory neurons that express the olfactory receptor Olfr17. |
Allele Name | targeted mutation 7, Peter Mombaerts |
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Allele Type | Targeted (Reporter) |
Allele Synonym(s) | P2-GFP; P2-IRES-tauGFP; P2-iTG |
Gene Symbol and Name | Olfr17, olfactory receptor 17 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | The tauGFP fusion protein is expressed in olfactory sensory neurons that express the olfactory receptor Olfr17. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 7 |
Molecular Note | An IRES-tauGFP was inserted 3 base pairs after the stop codon of the endogenous coding sequence yielding an insertional mutation that results in bicistronic expression of the endogenous gene and tauGFP. |
Mutations Made By | Peter Mombaerts, Max Planck Research Unit for Neurogenetics |
When using the P2-GFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #006669 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Olfr17<tm7Mom> |
Frozen Mouse Embryo | STOCK Olfr17<tm7Mom>/MomJ | $2595.00 |
Frozen Mouse Embryo | STOCK Olfr17<tm7Mom>/MomJ | $2595.00 |
Frozen Mouse Embryo | STOCK Olfr17<tm7Mom>/MomJ | $3373.50 |
Frozen Mouse Embryo | STOCK Olfr17<tm7Mom>/MomJ | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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