STOCK Or10a4^tm7Mom/MomJ

Stock number: 006669
Product name: P2-GFP
Strain synonyms: P2-IRES-tauGFP
Research areas: Research Tools, Sensorineural Research

Description

These GFP reporter mice are useful in studies to identify olfactory sensory neuronal development and topography in glomeruli in the olfactory bulb.

Detailed description

Olfactory sensory neurons that express the olfactory receptor Or10a4 (formerly Olfr17) also co-express the tauGFP fusion protein by virtue of IRES-mediated co-translation. These neurons can be revealed by the intrinsic fluorescence of GFP, or by immunofluorescence with anti-GFP antibodies.

Development

Homologous recombination to generate this allele was done in 129P2/OlaHsd derived E14 ES cells. To excise the loxP-flanked neomycin resistance sequence, the resulting mouse was bred with a mouse carrying an EIIa-cre transgene (Lakso et al. 1996, Proc Natl Acad Sci 93:5860), a transgene generated in FVB/N but having been backcrossed at least four generations to C57BL/6 before this cross and having unknown pedigree prior to that. The resulting mice, lacking the neomycin resistance cassette, were intercrossed and homozygotes were selected that were devoid of the EIIa-cre transgene.

Allele

Symbol: Or10a4^tm7Mom
Name: targeted mutation 7, Peter Mombaerts
MGI accession ID: MGI:3692295
Generation method: Targeted
Attributes: Reporter
Marker symbol: Or10a4
Marker name: olfactory receptor family 10 subfamily A member 4
Chromosome: 7
Strain of origin: 129P2/OlaHsd
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: The tauGFP fusion protein is expressed in olfactory sensory neurons that express the olfactory receptor Olfr17.
Molecular note: An IRES-tauGFP was inserted 3 base pairs after the stop codon of the endogenous coding sequence yielding an insertional mutation that results in bicistronic expression of the endogenous gene and tauGFP.