B6;129P2-Omp^tm4(cre)Mom/MomJ

Stock number: 006668
Product name: OMP-Cre
Research areas: Research Tools, Sensorineural Research

Description

These Cre recombinase mice are useful in studies to identify olfactory sensory neuronal lineage and topography in glomeruli in the olfactory bulb.

Detailed description

The coding region and part of the 3' non-translated region of the Omp gene was replaced by Cre. The targeted mutation results in a knockout. Mature olfactory sensory neurons express the Cre recombinase at high levels. Homozygous mice are subfertile.

As an example, when crossed to a strain with widespread expression of GFP and a loxP-flanked Bgeo reporter (see Stock No. 004178), this mutant mouse strain may be useful in lineage tracing.

View cre expression characterization.

Development

The 129P2/OlaHsd derived E14 ES cell was used to generate this targeted mutation and the resulting mice were crossed to C57BL/6, and then intercrossed to homozygosity. The FRT-flanked neo cassette (FNF) is retained within this strain, and can be removed by flp-mediated recombination.

The plasmid that was used to generate this mutation is banked and described at Addgene.

Allele

Symbol: Omp^tm4(cre)Mom
Name: targeted mutation 4, Peter Mombaerts
MGI accession ID: MGI:3692980
Generation method: Targeted
Attributes: Recombinase-expressing
Marker symbol: Omp
Marker name: olfactory marker protein
Chromosome: 7
Strain of origin: 129P2/OlaHsd
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: mature olfactory sensory neurons
Molecular note: Sequence encoding cre recombinase and a neomycin resistance cassette flanked by FRT sites was substituted in place of the Omp coding region.