B6;129P2-Omp^tm3Mom/MomJ

Stock number: 006667
Product name: OMP-GFP
Research areas: Research Tools, Sensorineural Research

Description

These GFP reporter mice are useful in studies to identify olfactory sensory neuronal development and topography in glomeruli in the olfactory bulb.

Detailed description

The coding region and part of the 3' non-translated region of the Omp gene was replaced by GFP. The targeted mutation results in a knockout. Mature olfactory sensory neurons express GFP at high levels. These neurons can be revealed by the intrinsic fluorescence of GFP, or by immunofluorescence with anti-GFP antibodies. Homozygous mice are subfertile.

Development

This mutation was generated in 129P2/OlaHsd derived E14 ES cells, the loxP flanked tk-neo cassette was excised by transient transfection of a plasmid expressing Cre recombinase, and the resulting mice were bred to C57BL/6 before intercrossing to homozygosity.

Allele

Symbol: Omp^tm3Mom
Name: targeted mutation 3, Peter Mombaerts
MGI accession ID: MGI:3692201
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Omp
Marker name: olfactory marker protein
Chromosome: 7
Strain of origin: 129P2/OlaHsd
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: Mature olfactory sensory neurons express GFP at high levels.
Molecular note: The coding sequence was replaced with a GFP-LTNL sequence and the LTNL cassette was recombined out through the transfection of the targeted ES cells with cre recombinase.