These GFP reporter mice are useful in studies to identify olfactory sensory neuronal development and topography in glomeruli in the olfactory bulb.
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Omp | olfactory marker protein |
The coding region and part of the 3' non-translated region of the Omp gene was replaced by GFP. The targeted mutation results in a knockout. Mature olfactory sensory neurons express GFP at high levels. These neurons can be revealed by the intrinsic fluorescence of GFP, or by immunofluorescence with anti-GFP antibodies. Homozygous mice are subfertile.
This mutation was generated in 129P2/OlaHsd derived E14 ES cells, the loxP flanked tk-neo cassette was excised by transient transfection of a plasmid expressing Cre recombinase, and the resulting mice were bred to C57BL/6 before intercrossing to homozygosity.
Expressed Gene | GFP, Green Fluorescent Protein, |
---|---|
Site of Expression | Mature olfactory sensory neurons express GFP at high levels. |
Allele Name | targeted mutation 3, Peter Mombaerts |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | OMP-GFP |
Gene Symbol and Name | Omp, olfactory marker protein |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | Mature olfactory sensory neurons express GFP at high levels. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 7 |
Molecular Note | The coding sequence was replaced with a GFP-LTNL sequence and the LTNL cassette was recombined out through the transfection of the targeted ES cells with cre recombinase. |
Mutations Made By | Peter Mombaerts, Max Planck Research Unit for Neurogenetics |
When using the OMP-GFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #006667 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Omp<tm3Mom> |
Frozen Mouse Embryo | B6;129P2-Omp<tm3Mom>/MomJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Omp<tm3Mom>/MomJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Omp<tm3Mom>/MomJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129P2-Omp<tm3Mom>/MomJ Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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