B6.129S6-Srf^tm1Rmn/J

Stock number: 006658
Product name: Srf^f
Research areas: Research Tools

Description

These floxed mutant mice possess loxP sites that flank promoter and exon 1 sequences of the Srf gene. This strain may be useful for generating conditional mutations in applications related to studies of cardiovascular disease.

Detailed description

These mice possess loxP sites that flank promoter and exon 1 sequences. Mice that are homozygous for this allele are viable and fertile. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Excision of the floxed fragment results in the removal of key regulatory elements and many of the DNA-binding and dimerization residues of the gene.

When bred to a strain expressing Cre recombinase under the control of mouse transgelin promoter (see Stock No. 004746 for example), this mutant mouse strain may be useful in studies of cardiovascular disease.

Development

loxP sites were inserted 1.4 kb upstream and 1.1 kb downstream of the transcription start site, resulting in floxed promoter and exon 1 sequences. The construct was electroporated into 129/Sv-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with C57BL/6.

Allele

Symbol: Srf^tm1Rmn
Name: targeted mutation 1, Narendrakumar Ramanan
MGI accession ID: MGI:3522206
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Srf
Marker name: serum response factor
Chromosome: 17
Strain of origin: 129S6/SvEvTac
Molecular note: LoxP sites were inserted to flank the promoter and exon 1, the region containing key regulatory elements important for promoter activity as well as many of the DNA-binding and dimerization residues.