These GFP reporter mice are useful in studies to identify olfactory sensory neuronal development and topography in glomeruli in the olfactory bulb.
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter) | Vmn2r26 | vomeronasal 2, receptor 26 |
An IRES-tauGFP-LNL sequence was introduced just downstream of the Vmn2r26 stop codon in 129P2/OlaHsd derived ES cell line E14. To excise the neomycin resistance sequence, the resulting mouse was bred with a mouse carrying Tg(EIIa-cre)C5379Lmgd, a transgene generated in FVB/N but having been backcrossed at least four generations to C57BL/6 before this cross and having unknown pedigree prior to that. The resulting loxP-positive F1 were intercrossed and Vmn2r26tm1Mom homozygotes were selected that were devoid of this cre transgene.
Expressed Gene | GFP, Green Fluorescent Protein, |
---|---|
Expressed Gene | Vmn2r26, vomeronasal 2, receptor 26, mouse, laboratory |
Site of Expression | GFP and Vmn2r26 are expressed in vomeronasal sensory neurons. |
Allele Name | targeted mutation 1, Peter Mombaerts |
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Allele Type | Targeted (Reporter) |
Allele Synonym(s) | V2r1b-IRES-tauGFP; V2R-GFP |
Gene Symbol and Name | Vmn2r26, vomeronasal 2, receptor 26 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | Vmn2r26, vomeronasal 2, receptor 26, mouse, laboratory |
Site of Expression | GFP and Vmn2r26 are expressed in vomeronasal sensory neurons. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 6 |
General Note | This is a GFP reporter gene for which monoallelic expression is found |
Molecular Note | IRES-tauGFP-LNL was inserted immediately after the V2r1b stop codon and the mice generated from the neomycin selected ES cells were bred to mice bearing Tg(EIIa-cre)C5379Lmgd to excise the neomycin-selectable marker leaving only loxP site. This targeted insertion results in cotranslation of tauGFP and V2R1Bfrom a bicistronic message. |
Mutations Made By | Peter Mombaerts, Max Planck Research Unit for Neurogenetics |
When using the V2R-GFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #006657 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Vmn2r26<tm1Mom> |
Frozen Mouse Embryo | STOCK Vmn2r26<tm1Mom>/MomJ | $2595.00 |
Frozen Mouse Embryo | STOCK Vmn2r26<tm1Mom>/MomJ | $2595.00 |
Frozen Mouse Embryo | STOCK Vmn2r26<tm1Mom>/MomJ | $3373.50 |
Frozen Mouse Embryo | STOCK Vmn2r26<tm1Mom>/MomJ | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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