STOCK Vmn2r26^tm1Mom/MomJ

Stock number: 006657
Product name: V2R-GFP
Strain synonyms: V2r1b-IRES-tauGFP
Research areas: Research Tools

Description

These GFP reporter mice are useful in studies to identify olfactory sensory neuronal development and topography in glomeruli in the olfactory bulb.

Development

An IRES-tauGFP-LNL sequence was introduced just downstream of the Vmn2r26 stop codon in 129P2/OlaHsd derived ES cell line E14. To excise the neomycin resistance sequence, the resulting mouse was bred with a mouse carrying Tg(EIIa-cre)C5379Lmgd, a transgene generated in FVB/N but having been backcrossed at least four generations to C57BL/6 before this cross and having unknown pedigree prior to that. The resulting loxP-positive F1 were intercrossed and Vmn2r26^tm1Mom homozygotes were selected that were devoid of this cre transgene.

Allele

Symbol: Vmn2r26^tm1Mom
Name: targeted mutation 1, Peter Mombaerts
MGI accession ID: MGI:3692027
Generation method: Targeted
Attributes: Reporter
Marker symbol: Vmn2r26
Marker name: vomeronasal 2, receptor 26
Chromosome: 6
Strain of origin: 129P2/OlaHsd
Expressed genes: GFP,Green Fluorescent Protein,; Vmn2r26,vomeronasal 2, receptor 26,mouse, laboratory
Site of expression: GFP and Vmn2r26 are expressed in vomeronasal sensory neurons.
Molecular note: IRES-tauGFP-LNL was inserted immediately after the V2r1b stop codon and the mice generated from the neomycin selected ES cells were bred to mice bearing Tg(EIIa-cre)C5379Lmgd to excise the neomycin-selectable marker leaving only loxP site. This targeted insertion results in cotranslation of tauGFP and V2R1Bfrom a bicistronic message.
General note: This is a GFP reporter gene for which monoallelic expression is found