B6;129P2-Or10j5^tm1Mom/MomJ

Stock number: 006642
Product name: MOR23-lacZ
Strain synonyms: MOR23-IRES-taulacZ

Development

The Or10j5^tm1Mom targeted mutation (formerly Olfr16^tm1Mom) was generated in 129P2/OlaHsd derived E14 ES cells, and the founder was bred to C57BL/6J. The loxP flanked neomycin resistance cassette was excised by breeding with a mouse carrying an EIIa-cre transgene (Lakso et al. 1996, Proceedings of the National Academy of Sciences, v93 p5860), a transgene generated in FVB/N but having been backcrossed at least four generations to C57BL/6 before this cross and having unknown pedigree prior to that. The resulting mice, lacking the neomycin resistance cassette, were intercrossed and homozygotes were selected that were devoid of the EIIa-cre transgene. In 2007 sperm were cryopreserved from homozygous males.

Allele

Symbol: Or10j5^tm1Mom
Name: targeted mutation 1, Peter Mombaerts
MGI accession ID: MGI:3051332
Generation method: Targeted
Attributes: Reporter
Marker symbol: Or10j5
Marker name: olfactory receptor family 10 subfamily J member 5
Chromosome: 1
Strain of origin: Not Specified
Expressed genes: lacZ,beta-galactosidase,E. coli
Molecular note: An IRES-taulacZ cassette was inserted downstream of the endogenous coding sequence to allow for coexpression of the gene and the marker. Southern blot confirmed recombination. Crossing with EIIa-Cre mice excised a floxed neo at the 3' end of the inserted cassette.