Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Reporter) | Olfr157 | olfactory receptor 157 |
This targeted mutation was generated in 129P2/OlaHsd derived E14 ES cells and the loxP-flanked neomycin (LTNL) cassette was excised by transient transfection of the targeted ES clones with a plasmid expressing cre recombinase. Recombinant clone V27/Cre1 was then injected into C57BL/6J blastocysts and the resulting mice were bred with C57BL/6J before intercrossing. In 2007 sperm was cryopreserved from homozygous males.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
---|---|
Site of Expression |
Allele Name | targeted mutation 1, Peter Mombaerts |
---|---|
Allele Type | Targeted (Reporter) |
Allele Synonym(s) | C-lacZ; mOR37C-IRES-taulacZ |
Gene Symbol and Name | Olfr157, olfactory receptor 157 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 4 |
Molecular Note | The gene was tagged by insertion of an IRES-lacZ-floxed neo cassette 3' to the stop codon. Transient expression of cre recombinase in correctly targeted ES cells removed the floxed neo cassette prior to blastocyst injection. Whole-mount histochemical analysis and in situ hybridization confirmed that the expression pattern of the tagged gene in the olfactory sensory neurons is similar to that of wild-type. |
Mutations Made By | Peter Mombaerts, Max Planck Research Unit for Neurogenetics |
When maintaining a live colony, homozygous mice may be bred.
When using the C-lacZ mouse strain in a publication, please cite the originating article(s) and include JAX stock #006640 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Olfr157<tm1Mom> |
Frozen Mouse Embryo | B6;129P2-Olfr157<tm1Mom>/MomJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Olfr157<tm1Mom>/MomJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Olfr157<tm1Mom>/MomJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129P2-Olfr157<tm1Mom>/MomJ Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.