B6;129P2-Or13c7c^tm1Mom/MomJ

Stock number: 006640
Product name: C-lacZ
Strain synonyms: mOR37C-IRES-taulacZ

Development

This targeted mutation was generated in 129P2/OlaHsd derived E14 ES cells and the loxP-flanked neomycin (LTNL) cassette was excised by transient transfection of the targeted ES clones with a plasmid expressing cre recombinase. Recombinant clone V27/Cre1 was then injected into C57BL/6J blastocysts and the resulting mice were bred with C57BL/6J before intercrossing. In 2007 sperm was cryopreserved from homozygous males.

Allele

Symbol: Or13c7c^tm1Mom
Name: targeted mutation 1, Peter Mombaerts
MGI accession ID: MGI:2385950
Generation method: Targeted
Attributes: Reporter
Marker symbol: Or13c7c
Marker name: olfactory receptor family 13 subfamily C member 7C
Chromosome: 4
Strain of origin: 129P2/OlaHsd
Expressed genes: lacZ,beta-galactosidase,E. coli
Molecular note: The gene was tagged by insertion of an IRES-lacZ-floxed neo cassette 3' to the stop codon. Transient expression of cre recombinase in correctly targeted ES cells removed the floxed neo cassette prior to blastocyst injection. Whole-mount histochemical analysis and in situ hybridization confirmed that the expression pattern of the tagged gene in the olfactory sensory neurons is similar to that of wild-type.