STOCK Vmn1r49^{tm4(Or8a1)Mom}/MomJ

Stock number: 006634
Product name: MV
Strain synonyms: M71->VRi2-IRES-taulacZ

Description

These LacZ reporter mice are useful in studies to identify olfactory sensory neuronal development and topography in glomeruli in the olfactory bulb.

Detailed description

Replacement of the coding region of the vomeronasal receptor Vmn1r49 (formerly called V1rb2) with that of the odorant receptor Or8a1 results in an alteration in which glomeruli the expressing axons converge upon in the accessory olfactory bulb. Vomeronasal sensory neurons that express the mutant locus co-express the taulacZ fusion protein by virtue of IRES-mediated co-translation. These neurons can be revealed by histochemistry of b-galactosidase enzymatic activity, or by immunofluorescence with anti-beta-galactosidase antibodies.

Development

An Or8a1-IRES-taulacZ-LNL sequence was introduced in place of the Vmn1r49 (formerly called V1rb2) coding sequence in 129P2/OlaHsd derived ES cell line E14. To excise the neomycin resistance sequence, the resulting mouse was bred with a mouse carrying Tg(EIIa-cre)C5379Lmgd, a transgene generated in FVB/N but having been backcrossed at least four generations to C57BL/6 before this cross and having unknown pedigree prior to that. The resulting mice, lacking the neomycin resistance cassette and retaining one loxP site, were intercrossed and Vmn1r49^{tm4(Or8a1)Mom} homozygotes were selected that were devoid of the EIIa-cre transgene.

Allele

Symbol: Vmn1r49^{tm4(Or8a1)Mom}
Name: targeted mutation 4, Peter Mombaerts
MGI accession ID: MGI:3640470
Generation method: Targeted
Marker symbol: Vmn1r49
Marker name: vomeronasal 1, receptor 49
Chromosome: 6
Strain of origin: 129P2/OlaHsd
Expressed genes: Or8a1,olfactory receptor family 8 subfamily A member 1,mouse, laboratory; lacZ,beta-galactosidase,E. coli
Molecular note: The coding region was replaced with that of Olfr151-IRES-taulacZ.