These LacZ reporter mice are useful in studies to identify olfactory sensory neuronal development and topography in glomeruli in the olfactory bulb.
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted | Vmn1r49 | vomeronasal 1, receptor 49 |
Replacement of the coding region of the vomeronasal receptor V1rb2 with that of the odorant receptor Olfr151 results in an alteration in which glomeruli the expressing axons converge upon in the accessory olfactory bulb. Vomeronasal sensory neurons that express the mutant locus co-express the taulacZ fusion protein by virtue of IRES-mediated co-translation. These neurons can be revealed by histochemistry of b-galactosidase enzymatic activity, or by immunofluorescence with anti-beta-galactosidase antibodies.
An Olfr151-IRES-taulacZ-LNL sequence was introduced in place of the V1rb2 coding sequence in 129P2/OlaHsd derived ES cell line E14. To excise the neomycin resistance sequence, the resulting mouse was bred with a mouse carrying Tg(EIIa-cre)C5379Lmgd, a transgene generated in FVB/N but having been backcrossed at least four generations to C57BL/6 before this cross and having unknown pedigree prior to that. The resulting mice, lacking the neomycin resistance cassette and retaining one loxP site, were intercrossed and V1rb2tm4Mom homozygotes were selected that were devoid of the EIIa-cre transgene.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Expressed Gene | Olfr151, olfactory receptor 151, mouse, laboratory |
Site of Expression |
Allele Name | targeted mutation 4, Peter Mombaerts |
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Allele Type | Targeted |
Allele Synonym(s) | M71->VRi2-IRES-taulacZ; MV; V1rb2mv |
Gene Symbol and Name | Vmn1r49, vomeronasal 1, receptor 49 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Expressed Gene | Olfr151, olfactory receptor 151, mouse, laboratory |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 6 |
Molecular Note | The coding region was replaced with that of Olfr151-IRES-taulacZ. |
When using the MV mouse strain in a publication, please cite the originating article(s) and include JAX stock #006634 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for V1rb2<tm4(Olfr151)Mom> |
Frozen Mouse Embryo | STOCK Vmn1r49<tm4(Olfr151)Mom>/MomJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Vmn1r49<tm4(Olfr151)Mom>/MomJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Vmn1r49<tm4(Olfr151)Mom>/MomJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Vmn1r49<tm4(Olfr151)Mom>/MomJ Frozen Embryo | $3373.50 |
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