STOCK Vmn1r49^tm1Mom/MomJ

Stock number: 006631
Product name: VL
Strain synonyms: VRi2-IRES-taulacZ
Research areas: Neurobiology Research, Research Tools

Description

These LacZ reporter mice are useful in studies to identify olfactory sensory neuronal development and topography in glomeruli in the olfactory bulb.

Detailed description

Vomeronasal sensory neurons that express the vomeronasal receptor V1rb2 also co-express the taulacZ fusion protein by virtue of IRES-mediated co-translation. These neurons can be revealed by histochemistry of beta-galactosidase enzymatic activity, or by immunofluorescence with anti-beta-galactosidase antibodies.

Development

An IRES-taulacZ-LNL sequence was introduced just downstream of the V1rb2 stop codon in 129P2/OlaHsd derived ES cell line E14. To excise the neomycin resistance sequence, the resulting mouse was bred with a mouse carrying Tg(EIIa-cre)C5379Lmgd, a transgene generated in FVB/N but having been backcrossed at least four generations to C57BL/6 before this cross and having unknown pedigree prior to that. The resulting mice, lacking the neomycin resistance cassette and retaining one loxP site, were intercrossed and V1rb2^tm1Mom homozygotes were selected that were devoid of the EIIa-cre transgene.

Allele

Symbol: Vmn1r49^tm1Mom
Name: targeted mutation 1, Peter Mombaerts
MGI accession ID: MGI:3640467
Generation method: Targeted
Attributes: Reporter
Marker symbol: Vmn1r49
Marker name: vomeronasal 1, receptor 49
Chromosome: 6
Strain of origin: 129P2/OlaHsd
Expressed genes: Vmn1r49,vomeronasal 1, receptor 49,mouse, laboratory; lacZ,beta-galactosidase,E. coli
Molecular note: An IRES-taulacZ sequence was inserted just downstream of the endogenous stop codon creating bicistronic co-expression of this marker and the endogenous message.