Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Reporter, Inserted expressed sequence) | Olfr151 | olfactory receptor 151 |
The Olfr151tm9Mom mutation was generated in 129P2/OlaHsd derived E14 ES cells. To excise the neomycin resistance sequence of the LNL cassette, a resulting mouse was bred with a mouse carrying Tg(EIIa-cre)Lmgd, a transgene generated in FVB/N but having been backcrossed at least 10 times to C57BL/6J before this cross. The transgene was then bred out of the line and the mice were intercrossed to homozygosity. In 2007 sperm was cryopreserved from homozygous males on this predominantly mixed B6;129P2 background.
The plasmid that was used to generate this mutation is banked and described at Addgene.
Expressed Gene | GFP, Green Fluorescent Protein, |
---|---|
Site of Expression | GFP is expressed in olfactory sensory neurons. |
Allele Name | targeted mutation 9, Peter Mombaerts |
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Allele Type | Targeted (Reporter, Inserted expressed sequence) |
Allele Synonym(s) | Hyb I; M71-M72 hybrid I |
Gene Symbol and Name | Olfr151, olfactory receptor 151 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | GFP is expressed in olfactory sensory neurons. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 9 |
Molecular Note | One bp change resulting in 1 amino acid substitution (A157T) and an IRES-tauGFP gene were introduced via homologous recombination. The resulting protein is a hybrid between Olfr151 and Olfr160 which have a total of 25 bp and 11 amino acid differences. The GFP gene was fused after the stop codon and co-expressed with the mutant protein. |
When using the STOCK Olfr151tm9Mom/MomJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #006623 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Olfr151<tm9Mom> |
Frozen Mouse Embryo | STOCK Olfr151<tm9Mom>/MomJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Olfr151<tm9Mom>/MomJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Olfr151<tm9Mom>/MomJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Olfr151<tm9Mom>/MomJ Frozen Embryo | $3373.50 |
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