STOCK Or8a1^tm9Mom/MomJ

Stock number: 006623
Strain synonyms: M71-M72 hybrid I

Development

The Or8a1^tm9Mom mutation (formerly Olfr151^tm9Mom) was generated in 129P2/OlaHsd derived E14 ES cells. To excise the neomycin resistance sequence of the LNL cassette, a resulting mouse was bred with a mouse carrying Tg(EIIa-cre)Lmgd, a transgene generated in FVB/N but having been backcrossed at least 10 times to C57BL/6J before this cross. The transgene was then bred out of the line and the mice were intercrossed to homozygosity. In 2007 sperm was cryopreserved from homozygous males on this predominantly mixed B6;129P2 background.

The plasmid that was used to generate this mutation is banked and described at Addgene.

Allele

Symbol: Or8a1^tm9Mom
Name: targeted mutation 9, Peter Mombaerts
MGI accession ID: MGI:3045148
Generation method: Targeted
Attributes: Reporter; Inserted expressed sequence
Marker symbol: Or8a1
Marker name: olfactory receptor family 8 subfamily A member 1
Chromosome: 9
Strain of origin: 129P2/OlaHsd
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: GFP is expressed in olfactory sensory neurons.
Molecular note: One bp change resulting in 1 amino acid substitution (A157T) and an IRES-tauGFP gene were introduced via homologous recombination. The resulting protein is a hybrid between Olfr151 and Olfr160 which have a total of 25 bp and 11 amino acid differences. The GFP gene was fused after the stop codon and co-expressed with the mutant protein.