B6;CBA-Tg(Thy1-CFP/COX8A)S2Lich/J

Stock number: 006617

Description

This strain has been discontinued. Please see Stock No. 007967.

Detailed description

This strain has been discontinued. Please see Stock No. 007967.

Allele

Symbol: Tg(Thy1-CFP/COX8A)S2Lich
Name: transgene insertion S2, Jeff W Lichtman
MGI accession ID: MGI:3715464
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Thy1-CFP/COX8A)S2Lich
Marker name: transgene insertion S2, Jeff W Lichtman
Chromosome: UN
Strain of origin: C57BL/6 and CBA
Expressed genes: COX8A,cytochrome c oxidase subunit 8A,human; CFP,Cyan Fluorescent Protein,jellyfish
Site of expression: mitochondria; neuronal populations including retinal ganglion cells, bipolar cells, amacrine cell and photoreceptors
Molecular note: A transgenic construct containing the entire open reading frame of the Cyan Fluorescent Protein (CFP) gene fused in-frame with the mitochondrial targeting sequence of the human cytochrome c oxidase subunit 8A (ubiquitous) gene under the direction of regulatory elements derived from the mouse Thy1 gene was injected into fertilized donor mouse eggs. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. The deleted sequences are required for expression in non-neural cells but not in neurons. The remainder of the sequence is required for neuronal expression. Founder line S2, corresponding to the published line S, was subsequently established.
General note: Founder line S2 corresponds to the published line S.