| Allele Name | transgene insertion S2, Jeff W Lichtman |
| Allele Type | Transgenic (Reporter) |
| Allele Synonym(s) | mitoCFP; MitoMouse; mtCFP; Thy1-CFP-MitoS; Thy1-mitoCFP-S; Thy1-mtCFP |
| Gene Symbol and Name | Tg(Thy1-CFP/COX8A)S2Lich, transgene insertion S2, Jeff W Lichtman |
| Gene Synonym(s) | |
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory |
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| Expressed Gene | COX8A, cytochrome c oxidase subunit 8A, human |
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| Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish |
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| Site of Expression | mitochondria; neuronal populations including retinal ganglion cells, bipolar cells, amacrine cell and photoreceptors |
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| Strain of Origin | C57BL/6 and CBA |
| Chromosome | UN |
| General Note | Founder line S2 corresponds to the published line S. |
| Molecular Note | A transgenic construct containing the entire open reading frame of the Cyan Fluorescent Protein (CFP) gene fused in-frame with the mitochondrial targeting sequence of the human cytochrome c oxidase subunit 8A (ubiquitous) gene under the direction of regulatory elements derived from the mouse Thy1 gene was injected into fertilized donor mouse eggs. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. The deleted sequences are required for expression in non-neural cells but not in neurons. The remainder of the sequence is required for neuronal expression. Founder line S2, corresponding to the published line S, was subsequently established. |
| Mutations Made By | Robert Burgess, The Jackson Laboratory |
