| Allele Name | transgene insertion 1, Corrinne Lobe |
| Allele Type | Transgenic (Conditional ready (e.g. floxed), Reporter, Inserted expressed sequence) |
| Allele Synonym(s) | Grg1 conditional transgene; Grg1lacZ; iZ/AP-Grg1; Tg(ACTB-Bgeo,-Tle1,-ALPP)1Lbe; Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe |
| Gene Symbol and Name | Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe, transgene insertion 1, Corrinne Lobe |
| Gene Synonym(s) | |
| Promoter | ACTB, actin, beta, chicken |
|---|
| Expressed Gene | ALPP, alkaline phosphatase, placental, human |
|---|
| Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli |
|---|
| Expressed Gene | lacZ, beta-galactosidase, E. coli |
|---|
| Site of Expression | lacZ activity is detected in all tissues tested including pancreas, lung, kidney, cerebellum, heart, muscle and lens and retina. Diminished activity is observed in liver tissue. When transgenic mice are crossed with a Cre recombinase-expressing strain, lacZ expression in the resulting offspring is replaced by both ALPP and TLE1 protein in cre-expressing tissues. |
|---|
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
| Chromosome | UN |
| Molecular Note | The transgene contains a strong cytomegalovirus (CMV) enhancer followed by the chicken beta-actin promoter; a loxP-flanked beta-galactosidase/neomycin resistance fusion (Bgeo) gene with three polyadenylation signals; the coding sequence of the mouse transducin-like enhancer of split 1, homolog of Drosophila E(spl) (Tle1) gene; and the human alkaline phosphatase, placental (Regan isozyme) (ALPP) gene preceded by an interal ribosome entry site (IRES). In mice doubly transgenic for this and a selected Cre recombinase (cre) transgene, excision of the floxed Bgeo results in whole-animal or tissue-specific co-expression of Tle1 and the ALPP reporter gene. |
