STOCK Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe/J

Stock number: 006613
Product name: Grg1^lacZ
Research areas: Cancer Research, Cell Biology Research, Developmental Biology Research, Research Tools

Description

In these transgenic mice co-expression of Tle1 and the human alkaline phosphatase (ALPP) reporter gene is blocked by the presence of a loxP-flanked Bgeo cassette inserted between the ACTB/CMV promoter and the Tle1 coding sequence.

Detailed description

Hemizygous transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the mouse transducin-like enhancer of split 1, homolog of Drosophila E(spl) gene, Tle1 (also known as Groucho-related gene 1; Grg1) under the regulation of the chicken beta actin promoter (ACTB) and the cytomegalovirus (CMV) intermediate-early enhancer. An internal ribosome entry sequence (IRES) followed by the human alkaline phosphatase (ALPP) reporter gene inserted downstream of the Tle1 gene directs co-expression of ALPP and TLE1. TLE1 and ALPP co-expression is blocked, however, by the presence of a loxP-flanked Bgeo (beta-galactosidase/neomycin resistance fusion protein) cassette inserted between the ACTB/CMV promoter and the Tle1 coding sequence. In the absence of Cre recombinase, beta-galactosidase activity is detected in all tissues tested including pancreas, lung, kidney, cerebellum, heart, muscle and lens and retina. Diminished beta-galactosidase activity is observed in liver tissue. When transgenic mice are crossed with a Cre recombinase-expressing strain, lacZ expression in the resulting offspring is replaced by both ALPP and TLE1 protein in cre-expressing tissues. These mice may be useful in studying lung tumorigenesis.

Development

A transgenic construct containing the human alkaline phosphatase, placental (Regan isozyme), gene ALPP, the mouse transducin-like enhancer of split 1, homolog of Drosophila E(spl) gene Tle1, loxP site flanked Bgeo reporter (beta-galactosidase/neomycin resistance fusion gene)/SV40 polyadenylation signal segment and IRES (Internal Ribosomal Entry) sequence under the control of the a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer, was introduced into R1 embryonic stem (ES) cells. ES cell clones containing a single copy of the construct were used to generate aggregated chimeric mice. Resulting male chimeric animals were bred with CD1 female mice.

Allele

Symbol: Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe
Name: transgene insertion 1, Corrinne Lobe
MGI accession ID: MGI:3696852
Generation method: Transgenic
Attributes: Conditional ready (e.g. floxed); Reporter; Inserted expressed sequence
Marker symbol: Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe
Marker name: transgene insertion 1, Corrinne Lobe
Chromosome: UN
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Expressed genes: ALPP,alkaline phosphatase, placental,human; Bgeo,fusion of beta-galactosidase and neomycin phosphotransferase genes,E. coli; lacZ,beta-galactosidase,E. coli
Site of expression: lacZ activity is detected in all tissues tested including pancreas, lung, kidney, cerebellum, heart, muscle and lens and retina. Diminished activity is observed in liver tissue. When transgenic mice are crossed with a Cre recombinase-expressing strain, lacZ expression in the resulting offspring is replaced by both ALPP and TLE1 protein in cre-expressing tissues.
Molecular note: The transgene contains a strong cytomegalovirus (CMV) enhancer followed by the chicken beta-actin promoter; a loxP-flanked beta-galactosidase/neomycin resistance fusion (Bgeo) gene with three polyadenylation signals; the coding sequence of the mouse transducin-like enhancer of split 1, homolog of Drosophila E(spl) (Tle1) gene; and the human alkaline phosphatase, placental (Regan isozyme) (ALPP) gene preceded by an interal ribosome entry site (IRES). In mice doubly transgenic for this and a selected Cre recombinase (cre) transgene, excision of the floxed Bgeo results in whole-animal or tissue-specific co-expression of Tle1 and the ALPP reporter gene.