In these transgenic mice co-expression of Tle1 and the human alkaline phosphatase (ALPP) reporter gene is blocked by the presence of a loxP-flanked Bgeo cassette inserted between the ACTB/CMV promoter and the Tle1 coding sequence.
Corrinne Lobe, Sunnybrook & Women's College HSC
Genetic Background | Generation |
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Allele Type |
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Transgenic (Conditional ready (e.g. floxed), Reporter, Inserted expressed sequence) |
Hemizygous transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the mouse transducin-like enhancer of split 1, homolog of Drosophila E(spl) gene, Tle1 (also known as Groucho-related gene 1; Grg1) under the regulation of the chicken beta actin promoter (ACTB) and the cytomegalovirus (CMV) intermediate-early enhancer. An internal ribosome entry sequence (IRES) followed by the human alkaline phosphatase (ALPP) reporter gene inserted downstream of the Tle1 gene directs co-expression of ALPP and TLE1. TLE1 and ALPP co-expression is blocked, however, by the presence of a loxP-flanked Bgeo (beta-galactosidase/neomycin resistance fusion protein) cassette inserted between the ACTB/CMV promoter and the Tle1 coding sequence. In the absence of Cre recombinase, beta-galactosidase activity is detected in all tissues tested including pancreas, lung, kidney, cerebellum, heart, muscle and lens and retina. Diminished beta-galactosidase activity is observed in liver tissue. When transgenic mice are crossed with a Cre recombinase-expressing strain, lacZ expression in the resulting offspring is replaced by both ALPP and TLE1 protein in cre-expressing tissues. These mice may be useful in studying lung tumorigenesis.
A transgenic construct containing the human alkaline phosphatase, placental (Regan isozyme), gene ALPP, the mouse transducin-like enhancer of split 1, homolog of Drosophila E(spl) gene Tle1, loxP site flanked Bgeo reporter (beta-galactosidase/neomycin resistance fusion gene)/SV40 polyadenylation signal segment and IRES (Internal Ribosomal Entry) sequence under the control of the a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer, was introduced into R1 embryonic stem (ES) cells. ES cell clones containing a single copy of the construct were used to generate aggregated chimeric mice. Resulting male chimeric animals were bred with CD1 female mice.
Expressed Gene | ALPP, alkaline phosphatase, placental, human |
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Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | lacZ activity is detected in all tissues tested including pancreas, lung, kidney, cerebellum, heart, muscle and lens and retina. Diminished activity is observed in liver tissue. When transgenic mice are crossed with a Cre recombinase-expressing strain, lacZ expression in the resulting offspring is replaced by both ALPP and TLE1 protein in cre-expressing tissues. |
Allele Name | transgene insertion 1, Corrinne Lobe |
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Allele Type | Transgenic (Conditional ready (e.g. floxed), Reporter, Inserted expressed sequence) |
Allele Synonym(s) | Grg1 conditional transgene; Grg1lacZ; iZ/AP-Grg1; Tg(ACTB-Bgeo,-Tle1,-ALPP)1Lbe; Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe |
Gene Symbol and Name | Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe, transgene insertion 1, Corrinne Lobe |
Gene Synonym(s) | |
Promoter | ACTB, actin, beta, chicken |
Expressed Gene | ALPP, alkaline phosphatase, placental, human |
Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | lacZ activity is detected in all tissues tested including pancreas, lung, kidney, cerebellum, heart, muscle and lens and retina. Diminished activity is observed in liver tissue. When transgenic mice are crossed with a Cre recombinase-expressing strain, lacZ expression in the resulting offspring is replaced by both ALPP and TLE1 protein in cre-expressing tissues. |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | UN |
Molecular Note | The transgene contains a strong cytomegalovirus (CMV) enhancer followed by the chicken beta-actin promoter; a loxP-flanked beta-galactosidase/neomycin resistance fusion (Bgeo) gene with three polyadenylation signals; the coding sequence of the mouse transducin-like enhancer of split 1, homolog of Drosophila E(spl) (Tle1) gene; and the human alkaline phosphatase, placental (Regan isozyme) (ALPP) gene preceded by an interal ribosome entry site (IRES). In mice doubly transgenic for this and a selected Cre recombinase (cre) transgene, excision of the floxed Bgeo results in whole-animal or tissue-specific co-expression of Tle1 and the ALPP reporter gene. |
When maintaining a live colony, these mice are bred as hemizygotes. The Donating Investigator maintained the strain by crossing hemizygote mice with wildtype mice.
When using the Grg1lacZ mouse strain in a publication, please cite the originating article(s) and include JAX stock #006613 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe |
Frozen Mouse Embryo | STOCK Tg(CAG-Bgeo -Tle1 -ALPP)1Lbe/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(CAG-Bgeo -Tle1 -ALPP)1Lbe/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(CAG-Bgeo -Tle1 -ALPP)1Lbe/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(CAG-Bgeo -Tle1 -ALPP)1Lbe/J Frozen Embryo | $3373.50 |
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