Biliary concentrations of phospholipids, cholesterol, and bile salts are reduced in Pctp (phosphatidylcholine transfer protein) homozygous mutants as compared to wildtype mice when fed a high fat, high cholesterol, cholate-containing lithogenic diet. There is a greater hepatic accumulation of phospholipid and cholesterol in the targeted mutant animals. On the high fat diet, HDL particles are of normal size, but plasma cholesterol and phospholipid concentrations are increased compared to wildtype mice. This strain represents a model that may be useful in studies of lipid metabolism.
David Cohen, Brigham and Women's Hospital
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Pctp | phosphatidylcholine transfer protein |
Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. Protein product from the targeted gene is not detected in the liver cytosol of 8-day-old homozygous pups. The lipid content and composition of bile and lung surfactant secretions are normal in homozygous targeted mice. Plasma cholesterol and phospholipid levels are not affected in chow-fed homozygous targeted mutation mice, but there is an increase in the accumulation of small alpha-migrating high density lipoprotein (HDL) particles. Biliary concentrations of phospholipids, cholesterol, and bile salts are reduced in homozygous mutants as compared to wildtype mice when fed a high fat, high cholesterol, cholate-containing lithogenic diet. There is a greater hepatic accumulation of phospholipid and cholesterol in the targeted mutant animals. On the high fat diet, HDL particles are of normal size, but plasma cholesterol and phospholipid concentrations are increased compared to wildtype mice. This strain represents a model that may be useful in studies of lipid metabolism.
A targeting vector containing a neomycin resistance gene was used to replace exons C and D by homologous recombination. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. The resultant strain has been backcrossed approximately twenty times to C57BL/6 by the donating laboratory.
Allele Name | targeted mutation 1, Piet Borst |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Pc-tp -; Pctp- |
Gene Symbol and Name | Pctp, phosphatidylcholine transfer protein |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 11 |
Molecular Note | A neomycin selection gene replaced exons C and D by homologous recombination. Western blot analysis demonstrated that no detectable protein product was made in the cytosol of cells derived from homozygous mice. |
Mutations Made By | Michele Wu, Brigham and Women's Hospital |
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the B6.129-Pctptm1Bor/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #006607 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Pctp<tm1Bor> |
Frozen Mouse Embryo | B6.129-Pctp<tm1Bor>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Pctp<tm1Bor>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Pctp<tm1Bor>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129-Pctp<tm1Bor>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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