B6.129-Pctp^tm1Bor/J

Stock number: 006607
Research areas: Metabolism Research

Description

Biliary concentrations of phospholipids, cholesterol, and bile salts are reduced in Pctp (phosphatidylcholine transfer protein) homozygous mutants as compared to wildtype mice when fed a high fat, high cholesterol, cholate-containing lithogenic diet. There is a greater hepatic accumulation of phospholipid and cholesterol in the targeted mutant animals. On the high fat diet, HDL particles are of normal size, but plasma cholesterol and phospholipid concentrations are increased compared to wildtype mice. This strain represents a model that may be useful in studies of lipid metabolism.

Detailed description

Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. Protein product from the targeted gene is not detected in the liver cytosol of 8-day-old homozygous pups. The lipid content and composition of bile and lung surfactant secretions are normal in homozygous targeted mice. Plasma cholesterol and phospholipid levels are not affected in chow-fed homozygous targeted mutation mice, but there is an increase in the accumulation of small alpha-migrating high density lipoprotein (HDL) particles. Biliary concentrations of phospholipids, cholesterol, and bile salts are reduced in homozygous mutants as compared to wildtype mice when fed a high fat, high cholesterol, cholate-containing lithogenic diet. There is a greater hepatic accumulation of phospholipid and cholesterol in the targeted mutant animals. On the high fat diet, HDL particles are of normal size, but plasma cholesterol and phospholipid concentrations are increased compared to wildtype mice. This strain represents a model that may be useful in studies of lipid metabolism.

Development

A targeting vector containing a neomycin resistance gene was used to replace exons C and D by homologous recombination. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. The resultant strain has been backcrossed approximately twenty times to C57BL/6 by the donating laboratory.

Allele

Symbol: Pctp^tm1Bor
Name: targeted mutation 1, Piet Borst
MGI accession ID: MGI:2673156
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Pctp
Marker name: phosphatidylcholine transfer protein
Chromosome: 11
Strain of origin: 129P2/OlaHsd
Molecular note: A neomycin selection gene replaced exons C and D by homologous recombination. Western blot analysis demonstrated that no detectable protein product was made in the cytosol of cells derived from homozygous mice.