This a variant of the protein tyrosine phosphatase type C receptor (Ptprc commonly called CD45.1/Ly5.1) on the BALB/cByJ background is useful in transplantation studies.
IMR Colony, The Jackson Laboratory
This is a congenic strain used in transplant studies because it carries the differential B cell antigen originally designated Ly5.1 and CD45. The current use of the Ptprc designation for Cd45 and Ly5 was based on work in humans following the report of Charbonneau and colleagues who first showed that a protein-tyrosine phosphatase (human placental protein-tyrosine phosphatase 1B) was homologous to the CD45 protein. Ptprc is one of a family of protein-tyrosine phosphatase genes involved in the regulation of cell growth. The b allele is normally present in the BALB and C57BL inbred strains.
This Ptprca allele was serially backcrossed for a minimum of 5 generations from B6.SJL-Ptprca Pepcb/ByJ (Stock No. 002014) onto BALB/cByJ (Stock No. 001026) with selection at each generation using a speed congenic protocol and identification of Ptprca by flow cytometry. This strain was generated by The Jackson Laboratory Repository in 2006.
|Allele Name||a variant|
|Allele Type||Not Applicable|
|Allele Synonym(s)||CD45.1; Ly5a; PtprcSJL|
|Gene Symbol and Name||Ptprc, protein tyrosine phosphatase, receptor type, C|
|Site of Expression||Widely expressed on all adaptive and innate immune cells.|
|Strain of Origin||Not Applicable|
|Molecular Note||Ptprca is found in strains SJL/J, STS/A, and DA. Ptprcb is found in strains C57BL/6, C3H/An, DBA/2, AKR, and many others (J:13367, J:12054, J:12077, J:8603). Twelve nucleotide differences between the a and b alleles have been identified. These base substitutions correspond to five amino-acid changes within the extracellular domain of the encoded protein. These amino-acid differences are clustered in a region that also contains the greatest divergence between mouse and rat sequences (J:22485). |
Note that the allele designations originally described were reversed in 1987 (J:8603); all publications prior to 1987 show SJL/J, STS/A, and DA as having the b allele and the C57BL/6J group as having the a allele (J:22341).
When maintaining a live colony, homozygous mice may be bred together.
When using the CByJ.SJL(B6)-Ptprca/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #006584 in your Materials and Methods section.